| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,548,257 | 0 | T | G | 53.3% | 7.9 / 13.1 | 15 | Y557S (TAC→TCC) | maeA | malate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (8/0); total (11/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGC > NZ_CP009273/1548174‑1548337 | gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTTGCGGGATTTgg > 2:463141/1‑90 (MQ=255)gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCgg > 2:182018/1‑90 (MQ=255)gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGTCGCGGGATTTgg > 2:361520/1‑90 (MQ=255)gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGGCGCGGGATTTgg > 1:225785/1‑90 (MQ=255) gagaGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGTCGCGGGATTCGGCTTTCCCGAAAtt > 1:104977/1‑90 (MQ=255) ccGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCt > 2:7142/1‑90 (MQ=255) cTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGGTTGCCAGAAATTATCGTCAATGGCCTGTTGCAgg > 2:508049/1‑90 (MQ=255) tCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCt > 1:56272/1‑80 (MQ=255) tCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCt < 2:56272/80‑1 (MQ=255) ccGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTTGACTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGGTTCGGc > 1:90203/1‑90 (MQ=255) gCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTc < 2:104977/90‑1 (MQ=255) aGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTCACCg > 2:32323/1‑90 (MQ=255) ggAGGTACGGCGGTAGTCGGGGTATTCGGCTTCCCAGAATTTCTCGTCAATGGCCTGTTGCGGGGCTTCGGCACAGGTTTTCCCCGCCAc < 1:463141/90‑1 (MQ=255) aCGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTCACCGCCACGCCTtg > 2:148298/1‑90 (MQ=255) tAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCGGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTtgctgctgc < 1:7142/90‑1 (MQ=255) | GATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGC > NZ_CP009273/1548174‑1548337 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |