| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,679,006 | 0 | A | C | 58.3% | 4.2 / 11.9 | 12 | D164A (GAC→GCC) | tus | DNA replication terminus site‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/7); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAA > NZ_CP009273/1678917‑1679090 | ccccACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGa > 2:461884/1‑90 (MQ=255) ccGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCg > 2:226656/1‑90 (MQ=255) ccgGCACGTTTTGAATGGGTGCATCGTCTTTTGCGGGGGCTGACCCCCTTTAATCCTTCCCCCCCCCCCCCCGTTCTCcccgcccccgcc < 1:603704/89‑1 (MQ=255) tttttGAAGGGGTGCCTCTTCTTTGGCGGGGGCTGACCCCCTTTATTGCTTCCCGCACCCCCCCCGTTCTGCCCGCCCCCGCCACTTTAc < 1:27733/89‑1 (MQ=255) tGAATGGGTGCATCGTCTTTTGCCGGGGCTGATCCCCCTTAATGCTTCCCGCCCGCTCCCCGTTCTGCACGCCCCCGCCACTTTACGCtt < 2:78679/90‑1 (MQ=255) gggTGCACCGTCATTTGCCGGGGCTCCCCCCCCTTAATGCTTCCCCCCCCCCCCCCGTTCTCCACGCCCCCGCCACTTTACGCTttggtt < 2:564515/90‑1 (MQ=255) gTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTttggttgg > 1:225786/1‑90 (MQ=255) tCGTCATTTGCCGGGGCTGATCCCCTTTAATGCTTCCCGCCCGCTCCCCGTTCTGCACGCCCCCGCCACTTTACGCTTTGGTTGGGCtaa < 2:318009/90‑1 (MQ=255) aTTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAAc > 1:323245/1‑90 (MQ=255) gCTGATCCCCTTTAATGCTTCCCCCCCGCCCCCCTTTCTCCACCCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTaa < 2:202561/90‑1 (MQ=255) gATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGaa > 1:43058/1‑90 (MQ=255) aaTGCTTCCCCCCCCCCCCCCTTTCTCCACGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGt < 2:221824/90‑1 (MQ=255) cTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGaa > 2:131381/1‑90 (MQ=255) gCACGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGaaaa < 2:225786/90‑1 (MQ=255) | CCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAA > NZ_CP009273/1678917‑1679090 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |