| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,336,782 | 0 | G | C | 53.3% | 12.1 / 19.2 | 15 | G289G (GGC→GGG) | yfaL | AIDA‑I family autotransporter adhesin YfaL/EhaC |
| Reads supporting (aligned to +/- strand): ref base G (6/1); new base C (0/8); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTC > NZ_CP009273/2336709‑2336871 | ctGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGagg > 1:211462/1‑90 (MQ=255) gttttGCCAAAAACCAGCTTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATAAAAAAACCCCCCCGCCGCAGaggaa < 1:138434/87‑1 (MQ=255) gTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGaggaa > 1:476844/1‑90 (MQ=255) gCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGAcc > 1:363829/1‑90 (MQ=255) gCCAAAACCCAGTTTTTTCCCGGCGGCAATATCAAAGGAAATTTCCCTTAACCCGAAAAACAAAAACCCCCCCGCCGCAGAGGAAGGAcc < 1:465423/90‑1 (MQ=255) aaaaaaCCAGCTTTTTCCCGCCGGCAATAGAAAAGGAACCTTCACTAAACCCGAAATAAAAAACCCCCCCCGCCGCAGAGGAAGGACCAt < 1:535778/86‑1 (MQ=255) aaTAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCAtc > 2:535778/1‑90 (MQ=255) aCCGGCTTTTTCCCGTCGGAAATATAAAAGGTAACTTCACTTAAGCCGAGATACAAAAAGCCCCCCGCCGCAGAGGAAGGACCATCTCCa < 2:476844/90‑1 (MQ=255) ttttCCGTCGACAATAACAAAGGAAACTTCCCTAAAGCCGAGAAACAAAAACCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCctgc < 1:94033/90‑1 (MQ=255) ttttCCGCCGGAAATAACAAAGGTAACTTCCCTAAAGCCGAAATACAAAAACCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCctgc < 1:553252/90‑1 (MQ=255) tttcCCGTCGGAAATATAAAAGGTAACTTCATTTAAGCCGAAATACAAAAACCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCctgc < 2:363829/90‑1 (MQ=255) tttcCCGCCGGAAATACCAAAGGTACCTCCACTAAAGCCAAAAAACAAAAACCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCctgc < 1:519147/90‑1 (MQ=255) tttCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCctgct > 2:491890/1‑90 (MQ=255) cccgccgGAAATATAAAAGGAAACTCCACTAAACCCGAAATAAAAAAACCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCtgctgc < 1:257800/89‑1 (MQ=255) agaTACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTgg > 2:171528/1‑90 (MQ=255) gCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGtc > 1:26651/1‑90 (MQ=255) | CTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTC > NZ_CP009273/2336709‑2336871 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |