| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,778,686 | 0 | T | G | 54.5% | 20.3 / 28.2 | 22 | intergenic (‑316/+435) | BW25113_RS25705/BW25113_RS13825 | DUF5507 domain‑containing protein/tRNA‑Ile |
| Reads supporting (aligned to +/- strand): ref base T (2/8); new base G (12/0); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.41e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTAC > NZ_CP009273/2778607‑2778756 | aTTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCa < 2:105140/90‑1 (MQ=255) ggTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCa < 2:282436/70‑1 (MQ=255) ggTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTTTGGTCAGTAGTAGATGTTTTAGGCgg > 2:453824/1‑89 (MQ=255) ggTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCa > 1:282436/1‑70 (MQ=255) ggTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGGCAGTAGTAGATGTTTAAGGCGt > 2:211920/1‑90 (MQ=255) aTGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTTTGGTCAGGAGTAGATGTTTTAAGCGGGGcgg > 1:323529/1‑88 (MQ=255) aTGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGGTCTTGGCAGTAGTAGATGTTTAAAGCGGGGccg > 1:468599/1‑88 (MQ=255) gCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTc < 2:367736/90‑1 (MQ=255) tGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGGTTTGGGCAGTAGTAGATGTATAAAGCGGGGGGGGGACAATTCATCCTTtctc > 1:367736/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCg < 1:45171/59‑1 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCg > 2:45171/1‑59 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGc > 2:50938/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGTTTTGGTCAGTAGTAGATGTTTTAAGCGGGGCAGAGACATTTTATCCTTTCTCTACGGc > 2:294682/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGTTTTGGTCAGTAGTAGATGTTTAAGGCGGGGGAGAGACATTTTATCCTTTCTCTACGGc > 1:169722/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGc > 2:294354/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGGTTTGGTCAGGAGTAGATGTTTTAGGCGGGGCGGAGACATTTTATCCTTTCTCTAccgg > 1:26313/1‑87 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGGTTTGGGCAGTAGGAGATGTTTTAGGGGGGGGAGAGGCAATTTATCCTTTCTCTACGGc > 1:58784/1‑90 (MQ=255) tCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCAt < 2:430594/90‑1 (MQ=255) tCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCAt < 1:50938/90‑1 (MQ=255) cacaAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCAGTAGGAGATGTTTAAAGCGTGGGAGAGACATTTCATCCTTTCTCTACGGCAtt > 1:565308/1‑90 (MQ=255) acaAAGCTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTCCTCTACGGCATTg < 2:495851/90‑1 (MQ=255) cTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCtac < 2:58784/90‑1 (MQ=255) | ATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTAC > NZ_CP009273/2778607‑2778756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |