| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,476,393 | 0 | C | G | 61.5% | 3.1 / 13.1 | 13 | A120G (GCG→GGG) | lptF | LPS export ABC transporter permease LptF |
| Reads supporting (aligned to +/- strand): ref base C (0/5); new base G (8/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGTGGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAAT > NZ_CP009273/4476306‑4476479 | gCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTTACGTGATGTgggggg > 2:206048/1‑90 (MQ=255) cTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGGTAAAGGGGTGggggggggggcg > 2:114446/1‑86 (MQ=255) gTTCTGGTGAAAGTGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGGTAAAGTGGTGTGGGGGGGGGCGGGGGGATcgcgg > 2:521470/1‑89 (MQ=255) ggTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGTGGGCGGGACCGTGGTCATCGCGtcatca < 2:550226/90‑1 (MQ=255) aaGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGGGATGTGGGGGGGGCCGGGGGCATCGCGGCATCAGGGTg > 2:199505/1‑90 (MQ=255) gCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGTGGGCGGGACCGTGGTCATCGCGTCATCAGGATGaa < 2:431056/90‑1 (MQ=255) ttGCGGTATTCACGGCAATCGTAGCGGCGGGTTAAGGGGTGTGGGGGGGGCCGTGGGCATCGCGGCCTCCGGGTGAAGTGTTTGCAGaag > 2:474820/1‑90 (MQ=255) aTTCACGGCAATCGTAGCGGCGGTTTAATTTGTGTGGGGGGGGCCGCGGGCAACGCGGGGCCAGGGCGGAGTGTTATAAGaaacggaagc > 1:453187/1‑90 (MQ=255) aTTCACGGCAATCGTAGCGGCGGTTAAAGTGATGTGGGGGGGGGCGTGGGCATCGCGTCCTTAGGATGGAGGGTTTGCAGaaaagaaaga > 2:294310/1‑89 (MQ=255) cGGCAATCGTAGCGGCGGTTAACGTGATGTGGGGGGGGCCGTGGGCATCGCGTCATCAGGGTGAAGGGTTTGCAGAagcgaaagcgaaac > 2:165411/1‑88 (MQ=255) aTCGTAGCGGCGGTTTACGTGATGTGGGGGGGGCCGGGGGCATCGCGGCATCAGGATGAAGGGTTAGGAGAAGCGGAAGCGAAACCCGGc > 2:234147/1‑90 (MQ=255) aTCGTAGCGGCGGTTAACGTGATGTGGGGGGGGCCGGGGGCATCGCGGCATCAGGATGAAGGGTTTGCAGAAAAGAAAACGAAaccccgc > 2:200042/1‑86 (MQ=255) cggTTAACGTGATGTGGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGgcgc < 1:217418/90‑1 (MQ=255) ggTTAACGTGATGTGGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCt < 2:453187/90‑1 (MQ=255) gggCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAAt < 1:204863/90‑1 (MQ=255) | GCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGTGGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAAT > NZ_CP009273/4476306‑4476479 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |