Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,106,357	T→G	A62A (GCA→GCC)	rfbB ←	dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,106,357	0	T	G	100.0%	24.5 / NA	8	A62A (GCA→GCC)	rfbB	dTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (5/3);  total (5/3)

ATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATCCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATT  >  NZ_CP009273/2106288‑2106421                                                                      |                                                                 aTGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATCCGTGCCATTGCAGGGGCATCGCAAATATCCGCATg                                              <  1:75385/90‑1 (MQ=255)       ttCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATCCGTGCCATTGCAGGGGCATCGCAAATATCCGCATGTTCaaa                                        >  2:440176/1‑90 (MQ=255)         cagcagCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATCCGTGCCATTGCAGGGGCAt                                                              <  1:478764/66‑1 (MQ=255)         cagcagCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATCCGTGCCATTGCAGGGGCAt                                                              >  2:478764/1‑66 (MQ=255)              gcCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATCCGTGCCATTGCAGGGGCATCGCAAATATCCGCATGTTCAAAAACATAg                                 >  2:214027/1‑90 (MQ=255)                             gCATCCGGCTGATGCTGAGCAAAAATCCGTGCCATTGCAGGGGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTtcagaatcagaa                  >  2:574499/1‑90 (MQ=255)                                        aTGCTGAGCAAAAATCCGTGCCATTGCAGGGGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAg       <  1:574499/90‑1 (MQ=255)                                             gAGCAAAAATCCGTGCCATTGCAGGGGTATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGAtt  >  1:30690/1‑90 (MQ=255)                                                                      |                                                                 ATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATCCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATT  >  NZ_CP009273/2106288‑2106421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑