Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,243,538	A→G	T115A (ACC→GCC)	yeiH →	YeiH family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,243,538	0	A	G	100.0%	28.8 / NA	9	T115A (ACC→GCC)	yeiH	YeiH family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/5);  total (4/5)

TCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAG  >  NZ_CP009273/2243453‑2243620                                                                                      |                                                                                   tCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTGCCtt                                                                                >  2:447143/1‑90 (MQ=255)    ttATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTGCCTTCct                                                                             >  2:324516/1‑90 (MQ=255)      aTGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTGCCTTCctgc                                                                           <  1:6900/90‑1 (MQ=255)                                   gCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTGCCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGttt                                              <  1:482981/90‑1 (MQ=255)                                        tGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTGCCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCt                                         <  2:464149/90‑1 (MQ=255)                                           cGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTGCCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGa                                      <  2:330404/90‑1 (MQ=255)                                                  aGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTGCCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGcac                               <  1:5283/90‑1 (MQ=255)                                                           atcatTGACGTGTTGACGCTGTCCAGTGCCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTgg                      >  2:351969/1‑90 (MQ=255)                                                                               gTCCAGTGCCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAg  >  1:192548/1‑90 (MQ=255)                                                                                      |                                                                                   TCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAG  >  NZ_CP009273/2243453‑2243620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑