Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,366,540	A→G	Y57C (TAT→TGT)	arnF →	4‑amino‑4‑deoxy‑L‑arabinose‑phosphoundecaprenol flippase subunit ArnF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,366,540	0	A	G	100.0%	72.1 / NA	21	Y57C (TAT→TGT)	arnF	4‑amino‑4‑deoxy‑L‑arabinose‑phosphoundecaprenol flippase subunit ArnF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/15);  total (6/15)

GGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGC  >  NZ_CP009273/2366471‑2366626                                                                      |                                                                                       gggATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTttgtt                                                                    <  1:171789/90‑1 (MQ=255)    aTTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGt                                                                 >  2:53667/1‑90 (MQ=255)    aTTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGt                                                                 >  2:110165/1‑90 (MQ=255)      tttATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGtat                                                               >  1:137589/1‑90 (MQ=255)      tttATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGtat                                                               >  2:522425/1‑90 (MQ=255)            gcgGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACg                                                         >  1:519748/1‑90 (MQ=255)               gCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCtt                                                      <  1:372120/90‑1 (MQ=255)                            ttGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTgcgc                                         <  2:174958/90‑1 (MQ=255)                             tGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCt                                        <  2:19887/90‑1 (MQ=255)                             tGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCt                                        <  2:50689/90‑1 (MQ=255)                                         ccGGATACTGTTACTCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTa                            <  1:110165/90‑1 (MQ=255)                                                   ttACTCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTc                  <  2:134335/90‑1 (MQ=255)                                                      cTCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCt                       <  1:594779/82‑1 (MQ=255)                                                      cTCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCt                       >  2:594779/1‑82 (MQ=255)                                                       tCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTa              <  1:53667/90‑1 (MQ=255)                                                       tCGGGCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTa              <  1:365850/90‑1 (MQ=255)                                                         gggCTGCTGGGTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATg            <  2:162277/90‑1 (MQ=255)                                                                   gTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGc  <  1:468590/90‑1 (MQ=255)                                                                   gTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGc  <  2:260177/90‑1 (MQ=255)                                                                   gTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGc  <  2:473196/90‑1 (MQ=255)                                                                   gTTGTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGc  <  1:273957/90‑1 (MQ=255)                                                                      |                                                                                       GGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGC  >  NZ_CP009273/2366471‑2366626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑