Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,753,169	T→C	S276P (TCA→CCA)	yfjI →	YfjI family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,753,169	0	T	C	94.1%	50.9 / ‑3.2	17	S276P (TCA→CCA)	yfjI	YfjI family protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (8/8);  total (8/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

AAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTT‑CAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCA  >  NZ_CP009273/2753085‑2753226                                                                                     |                                                           aaGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTC‑CAAAt                                                      >  1:372684/1‑90 (MQ=255)       attggattTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTC‑CAAATGAACAt                                                <  1:324151/90‑1 (MQ=255)       attggattTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTC‑CAAATGAACAt                                                >  2:175337/1‑90 (MQ=255)             ttttttGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTC‑CAAATGAACATTTGCCg                                          <  1:381214/90‑1 (MQ=255)              tttttGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTTCCAAATGAACATTTGCCg                                          <  2:156572/90‑1 (MQ=255)                        gTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTC‑CAAATGAACATTTGCCGGTATTTCACCa                               >  1:537594/1‑90 (MQ=255)                                  tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTC‑CAAATGAACATTTGCCGGTATTTCACCAACGTCTTATg                     >  1:391781/1‑90 (MQ=255)                                  tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTC‑CAAATGAACATTTGCCGGTATTTCACCAACGTCTTATg                     >  2:169917/1‑90 (MQ=255)                                  tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTC‑CAAATGAACATTTGCCGGTATTTCACCAACGTCTTATg                     >  1:85473/1‑90 (MQ=255)                                     cAGCCTGCTTCAACTCAAGGTAACAGAAAAATTTCCAACCCAATTTTTC‑CAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGaa                  <  2:539154/90‑1 (MQ=255)                                       gCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTC‑CAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAAt                <  1:69047/90‑1 (MQ=255)                                       gCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTC‑CAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAAt                <  1:175337/90‑1 (MQ=255)                                         cTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTC‑CAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTg              <  2:372684/90‑1 (MQ=255)                                          tGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTC‑CAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGt             <  2:537594/90‑1 (MQ=255)                                                     aaGGTAACAGAAAAATTTCCAACCCAATTTTTC‑CAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGca  >  1:548202/1‑90 (MQ=255)                                                                aaaaTTTCCAACCCAATTTTTC‑CAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATgag      <  1:422247/75‑1 (MQ=255)                                                                aaaaTTTCCAACCCAATTTTTC‑CAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATgag      >  2:422247/1‑75 (MQ=255)                                                                                     |                                                           AAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTT‑CAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCA  >  NZ_CP009273/2753085‑2753226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑