Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,763,617	A→G	I186V (ATT→GTT)	yfjR →	WYL domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,763,617	0	A	G	100.0%	55.4 / NA	16	I186V (ATT→GTT)	yfjR	WYL domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/6);  total (10/6)

CTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTT  >  NZ_CP009273/2763553‑2763696                                                                 |                                                                                cTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGGTTCAGCCTTTGCAGGAGACTTTTcg                                                        <  1:245593/90‑1 (MQ=255)          ggtggCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGGTTCAGCCTTTGCAGGAGACTTTTCGCCGCaa                                                  <  1:589881/87‑1 (MQ=255)          ggtggCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGGTTCAGCCTTTGCAGGAGACTTTTCGCCGCaa                                                  >  2:589881/1‑87 (MQ=255)          ggtggCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGGTTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGa                                               >  1:249103/1‑90 (MQ=255)          ggtggCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGGTTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGa                                               >  2:329563/1‑90 (MQ=255)             ggCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGGTTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAg                                            >  1:62211/1‑90 (MQ=255)             ggCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGGTTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAg                                            >  2:515318/1‑90 (MQ=255)                                atCGCCGTATTCACACTTGATGAAATCCATCTGGTTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGaa                         >  1:321390/1‑90 (MQ=255)                                    ccGTATTCACACTTGATGAAATCCATCTGGTTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCt                                >  1:70447/1‑79 (MQ=255)                                    ccGTATTCACACTTGATGAAATCCATCTGGTTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCt                                <  2:70447/79‑1 (MQ=255)                                    ccGTATTCACACTTGATGAAATCCATCTGGTTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGAcc                     >  2:500225/1‑90 (MQ=255)                                     cGTATTCACACTTGATGAAATCCATCTGGTTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGAccc                    <  2:321390/90‑1 (MQ=255)                                           cacaCTTGATGAAATCCATCTGGTTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCtt              <  1:500225/90‑1 (MQ=255)                                                  gatgaAATCCATCTGGTTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAg       >  2:351685/1‑90 (MQ=255)                                                       aaTCCATCTGGTTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCtt  >  1:345228/1‑90 (MQ=255)                                                       aaTCCATCTGGTTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCtt  <  2:209030/90‑1 (MQ=255)                                                                 |                                                                                CTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTT  >  NZ_CP009273/2763553‑2763696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑