| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,224,102 | T→C | F619S (TTC→TCC) | ygjK → | alpha‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,224,102 | 0 | T | C | 100.0% | 59.6 / NA | 17 | F619S (TTC→TCC) | ygjK | alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/10); total (7/10) | |||||||||||
GCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTT > NZ_CP009273/3224017‑3224184 | gCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTCCGAc < 2:129481/90‑1 (MQ=255)gCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTCCGAc < 2:556256/90‑1 (MQ=255) aGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTCCGACCCGACTACCCAGTTct > 1:99744/1‑90 (MQ=255) ggCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTCCGAc < 1:462893/64‑1 (MQ=255) ggCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTCCGAc > 2:462893/1‑64 (MQ=255) cGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACGCCTGTATGTCCGACCCGACTACCCAGTTCTACTATGACGTACGTAtt < 1:483821/90‑1 (MQ=255) cGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTCCGACCCGACTACCCAGTTCTACTATGACGTACGTAtt < 1:155270/90‑1 (MQ=255) gCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTCCGACCCGACTACCCAGTTCTACTATGACGTACGTATTg < 1:345601/90‑1 (MQ=255) cGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTCCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGAt < 1:477379/90‑1 (MQ=255) ccAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTCCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATaa < 2:98283/90‑1 (MQ=255) gACTACATCAACACCTGTATGTCCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGcgcg > 1:349415/1‑90 (MQ=255) aTCAACACCTGTATGTCCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCaaa < 2:99744/90‑1 (MQ=255) acacCTGTATGTCCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAAcc < 1:253515/61‑1 (MQ=255) acacCTGTATGTCCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAAcc > 2:253515/1‑61 (MQ=255) acacCTGTATGTCCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGa > 2:495965/1‑90 (MQ=255) cTGTATGTCCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGAttgt > 1:319084/1‑90 (MQ=255) tGTATGTCCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGAttgtt > 1:80248/1‑90 (MQ=255) | GCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTT > NZ_CP009273/3224017‑3224184 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |