Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,576,634	A→G	R79R (CGA→CGG)	BW25113_RS17825 →	YrhA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,576,634	0	A	G	100.0%	35.9 / NA	11	R79R (CGA→CGG)	BW25113_RS17825	YrhA family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/2);  total (9/2)

TAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCAT  >  NZ_CP009273/3576556‑3576717                                                                               |                                                                                    tAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCct                                                                          >  1:51013/1‑90 (MQ=255)          ttttAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTcc                                                                 >  1:3248/1‑90 (MQ=255)             tAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGa                                                              >  1:250375/1‑90 (MQ=255)             tAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGa                                                              >  1:472329/1‑90 (MQ=255)                  aTCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCtt                                                         >  2:124955/1‑90 (MQ=255)                                       ttttAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCg                                    <  1:58947/90‑1 (MQ=255)                                                         gatTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGaa                  >  1:454484/1‑90 (MQ=255)                                                         gatTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGaa                  >  2:356752/1‑90 (MQ=255)                                                                   tAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAAtgatga        >  1:180704/1‑90 (MQ=255)                                                                     aTGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGAtt      >  2:579315/1‑90 (MQ=255)                                                                         aTTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCAt  <  2:443734/90‑1 (MQ=255)                                                                               |                                                                                    TAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCAT  >  NZ_CP009273/3576556‑3576717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑