Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,715,736	T→C	K674K (AAA→AAG)	glyS ←	glycine‑‑tRNA ligase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,715,736	0	T	C	100.0%	144.7 / NA	39	K674K (AAA→AAG)	glyS	glycine‑‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/17);  total (22/17)

CCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAAGCATCAACCGG  >  NZ_CP009273/3715653‑3715819                                                                                    |                                                                                    ccTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTcc                                                                               >  1:291202/1‑90 (MQ=255)          tGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTc                                                                      <  1:125480/90‑1 (MQ=255)          tGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTc                                                                      <  2:217808/90‑1 (MQ=255)             cAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCCTCTCCAGCATGGTCAGa                                                                   <  2:174647/90‑1 (MQ=255)               ggCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACg                                                                 <  1:110282/90‑1 (MQ=255)               ggCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACg                                                                 >  2:150466/1‑90 (MQ=255)                    tttAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGa                                                            <  2:470875/90‑1 (MQ=255)                    tttAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGa                                                            <  1:311300/90‑1 (MQ=255)                           aCGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGa                                                                   <  2:173798/76‑1 (MQ=255)                           aCGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGa                                                                   >  1:173798/1‑76 (MQ=255)                           aCGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCaa                                                     >  1:444107/1‑90 (MQ=255)                           aCGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCaa                                                     >  2:164543/1‑90 (MQ=255)                             ggCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGa                                                                   >  2:593667/1‑74 (MQ=255)                             ggCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGa                                                                   <  1:593667/74‑1 (MQ=255)                             ggCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAAtt                                                   >  1:460491/1‑90 (MQ=255)                             ggCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAAtt                                                   >  2:589694/1‑90 (MQ=255)                                      attGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGtcat                                          >  1:448980/1‑90 (MQ=255)                                       ttGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGtcatc                                         <  1:553317/90‑1 (MQ=255)                                       ttGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGtcatc                                         <  1:594640/90‑1 (MQ=255)                                       ttGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGtcatc                                         <  1:164543/90‑1 (MQ=255)                                        tGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGtcatca                                        >  2:367075/1‑90 (MQ=255)                                             cAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCAt                                   <  1:199662/90‑1 (MQ=255)                                                 gAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGAcc                               >  2:303055/1‑90 (MQ=255)                                                 gAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGAcc                               <  2:192432/90‑1 (MQ=255)                                                 gAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGAcc                               >  1:192432/1‑90 (MQ=255)                                                                         aGTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAAGCATCa       >  1:311871/1‑90 (MQ=255)                                                                          gTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAAGCATCaa      >  2:263754/1‑90 (MQ=255)                                                                          gTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAAGCATCaa      >  1:577079/1‑90 (MQ=255)                                                                          gTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAAGCATCaa      >  2:325527/1‑90 (MQ=255)                                                                          gTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAAGCATCaa      >  2:359682/1‑90 (MQ=255)                                                                          gTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAAGCATCaa      >  2:495150/1‑90 (MQ=255)                                                                          gTTCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAAGCATCaa      >  2:526004/1‑90 (MQ=255)                                                                           ttCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAAGCATCAAc     <  2:291202/90‑1 (MQ=255)                                                                           ttCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAAGCATCAAc     <  2:444107/90‑1 (MQ=255)                                                                           ttCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAAGCATCAAc     <  2:524955/90‑1 (MQ=255)                                                                            tCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGc                                                       >  2:180009/1‑39 (MQ=37)                                                                            tCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGc                                                       <  1:180009/39‑1 (MQ=255)                                                                            tCGCGCAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAAGCATCAAcc    >  2:384460/1‑90 (MQ=255)                                                                              gcgcAGCTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAAGCATCAACCgg  >  2:458421/1‑90 (MQ=255)                                                                                    |                                                                                    CCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAAGCATCAACCGG  >  NZ_CP009273/3715653‑3715819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑