Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,717,969 T→C E237G (GAA→GGA)  glyQ ← glycine‑‑tRNA ligase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,717,9690TC91.7% 72.9 / ‑1.3 24E237G (GAA→GGA) glyQglycine‑‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (15/7);  total (16/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGTCCACATCCGCGTATTCGA  >  NZ_CP009273/3717889‑3718047
                                                                                |                                                                              
tGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCca                                                                       <  2:310574/90‑1 (MQ=255)
  gCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCcagc                                                                     >  1:356799/1‑90 (MQ=255)
      tACGCGCATCCAGTAGGTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAgct                                                                 >  2:508736/1‑90 (MQ=255)
                     ggTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCttctttct                                                  >  2:397754/1‑90 (MQ=255)
                          aaGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTAc                                             <  2:356799/90‑1 (MQ=255)
                          aaGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTAc                                             >  2:34093/1‑90 (MQ=255)
                              tgtgGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCt                                         >  2:294936/1‑90 (MQ=255)
                                tgGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCg                                       >  2:565805/1‑90 (MQ=255)
                                tgGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCg                                       >  1:510108/1‑90 (MQ=255)
                                    cggcTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGc                                   <  1:284921/90‑1 (MQ=255)
                                      gcTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAg                                 >  2:389092/1‑90 (MQ=255)
                                                tACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGa                       <  1:294936/90‑1 (MQ=255)
                                                tACGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGa                       >  1:578441/1‑90 (MQ=255)
                                                  cGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAg                     >  2:231224/1‑90 (MQ=255)
                                                  cGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAg                     >  2:202635/1‑90 (MQ=255)
                                                  cGCTCGTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAg                     >  2:165144/1‑90 (MQ=255)
                                                       gTAGGCTGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGTCcac                >  2:428250/1‑90 (MQ=255)
                                                             tGGCAGCGGCAGCGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTTGAAGCAGGTGAACAGGAGGTCCACATCcgc          <  1:45492/90‑1 (MQ=255)
                                                              ggcagcggcagcGGATTTCCCAGCGCCAGCAGCTGCTGCGcttc                                                       >  2:82419/1‑44 (MQ=255)
                                                              ggcagcggcagcGGATTTCCCAGCGCCAGCAGCTGCTGCGcttc                                                       >  2:282773/1‑44 (MQ=255)
                                                              ggcagcggcagcGGATTTCCCAGCGCCAGCAGCTGCTGCGcttc                                                       <  1:82419/44‑1 (MQ=255)
                                                              ggcagcggcagcGGATTTCCCAGCGCCAGCAGCTGCTGCGcttc                                                       <  1:282773/44‑1 (MQ=255)
                                                              ggcagcggcagcGGATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGTCCACATCcgcg         >  1:219599/1‑90 (MQ=255)
                                                                     gcagcggATTTCCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGTCCACATCCGCGTATTCGa  <  2:219599/90‑1 (MQ=255)
                                                                                |                                                                              
TGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGTCCACATCCGCGTATTCGA  >  NZ_CP009273/3717889‑3718047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑