Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,918,222	T→G	N258H (AAC→CAC)	mnmG ←	tRNA uridine‑5‑carboxymethylaminomethyl(34) synthesis enzyme MnmG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,918,222	0	T	G	100.0%	131.5 / NA	37	N258H (AAC→CAC)	mnmG	tRNA uridine‑5‑carboxymethylaminomethyl(34) synthesis enzyme MnmG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (20/17);  total (20/17)

CGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTGGGGATGCTGGGACGCATTGCCCAT  >  NZ_CP009273/3918153‑3918306                                                                      |                                                                                     cgatcgaCGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATc                                                                            >  2:389377/1‑80 (MQ=255) cgatcgaCGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATc                                                                            <  1:389377/80‑1 (MQ=255) cgatcgaCGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATggg                                                                  >  1:127987/1‑90 (MQ=255)      gaCGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTtc                                                             >  1:246701/1‑90 (MQ=255)      gaCGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTtc                                                             >  1:472184/1‑90 (MQ=255)      gaCGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTtc                                                             <  1:599997/90‑1 (MQ=255)          ggCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGt                                                         <  2:41524/90‑1 (MQ=255)               tAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTa                                                    <  2:440214/90‑1 (MQ=255)               tAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTa                                                    <  1:190723/90‑1 (MQ=255)               tAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTa                                                    <  2:332238/90‑1 (MQ=255)               tAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTa                                                    <  2:246701/90‑1 (MQ=255)                 gCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATg                                                  >  2:186503/1‑90 (MQ=255)                    tGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGt                                               <  2:529671/90‑1 (MQ=255)                    tGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGt                                               <  2:396552/90‑1 (MQ=255)                     gggCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTg                                              >  1:124462/1‑90 (MQ=255)                      ggCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGa                                             >  1:291051/1‑90 (MQ=255)                           acacCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATc                                                                            <  1:267250/54‑1 (MQ=255)                           acacCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATc                                                                            >  2:267250/1‑54 (MQ=255)                                ttCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGt                                                         >  2:4419/1‑68 (MQ=255)                                ttCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGt                                                         <  1:4419/68‑1 (MQ=255)                                gtccaTCACCCCTGCGTTTTTTGTTCTCCGATCGAGGTGACTGCGGATCACATAATTCCGTTTCTCGTTGGTATGAGTGATATACCACgg                                   >  1:175257/5‑90 (MQ=255)                                       aCCCCTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACctgc                            <  1:2142/90‑1 (MQ=255)                                        ccccTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATg                                                                    <  2:448180/49‑1 (MQ=255)                                        ccccTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATg                                                                    >  1:448180/1‑49 (MQ=255)                                         cccTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACctgctg                          <  2:291051/90‑1 (MQ=255)                                           cTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTtct                                                            <  1:476102/54‑1 (MQ=255)                                           cTGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTtct                                                            >  2:476102/1‑54 (MQ=255)                                            tGCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTgggg                       <  2:243448/90‑1 (MQ=255)                                             gCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTGGGGa                      >  2:460469/1‑90 (MQ=255)                                             gCGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTGGGGa                      >  1:56016/1‑90 (MQ=255)                                              cGTACATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTGGGGAt                     >  2:310359/1‑90 (MQ=255)                                                  cATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTGGGGATGCTg                 >  1:594671/1‑90 (MQ=255)                                                  cATTGGGCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTGGGGATGCTg                 >  2:136486/1‑90 (MQ=255)                                                       ggCTACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTGGGGATGCTGGGACg            >  1:81868/1‑90 (MQ=255)                                                          tACGATCGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTGGGGATGCTGGGACGCAt         <  2:212416/90‑1 (MQ=255)                                                                cGAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTGGGGATGCTGGGACGCATTGCCCa   >  2:147465/1‑90 (MQ=255)                                                                 gAGGTGACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTGGGGATGCTGGGACGCATTGCCCAt  >  2:122754/1‑90 (MQ=255)                                                                      |                                                                                     CGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTGGGGATGCTGGGACGCATTGCCCAT  >  NZ_CP009273/3918153‑3918306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑