| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,153,816 | T→C | S84P (TCA→CCA) | btuB → | TonB‑dependent vitamin B12 receptor BtuB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,153,816 | 0 | T | C | 94.7% | 59.9 / ‑4.1 | 19 | coding (250/1845 nt) | btuB | TonB‑dependent vitamin B12 receptor BtuB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (8/10); minor base . (1/0); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGT > NZ_CP009273/4153744‑4153899 | aCCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTcgc < 2:598736/90‑1 (MQ=255) gtgCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTTAGGTCAGCTCCCCTCTTTTTTTTTTTGCGGGACAAAAGCCAgg > 1:130810/1‑89 (MQ=255) gctgcGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCa < 1:598229/90‑1 (MQ=255) ctgcGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGGCAGC‑‑‑‑‑TCTATTTTTTTTCGCGGTACAAAAGCCAGTCATGTGtt > 1:147919/1‑90 (MQ=255) gcgcCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTTTTCGCGGTACAAAAGCCAGTCAtgt > 1:169761/1‑90 (MQ=255) gcgcCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCAtgt > 2:493481/1‑90 (MQ=255) cGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTg < 1:562706/90‑1 (MQ=255) gTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAAAGCCAGTCATGTGTTgg > 2:130431/1‑90 (MQ=255) ttCCGGGCGTCGATATCACACAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGgtgt < 1:104571/90‑1 (MQ=255) gggCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAAt < 1:188135/90‑1 (MQ=255) cGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCCTCTATTTTTTTTTGCGGGACAAAAGCCAGTCATGTGTTGGGGTTAAttta > 1:176267/1‑88 (MQ=255) gTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTTTTCGCGGGACAAATGCCAGTCATGTGTTGGGGTTAATTGAt > 2:188135/1‑90 (MQ=255) gTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGAt < 1:539755/90‑1 (MQ=255) atCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTa < 1:176815/90‑1 (MQ=255) cccAAAACGGCGGTTCAGGTCAGCTCCCCTCTATTTTTTTTTGCGGGACAAAAGCCAGTCATGGGTTGGGGTTTATTTATGGCGTACGcc > 1:257129/1‑90 (MQ=255) gTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCgg < 1:493481/90‑1 (MQ=255) cAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGt < 1:232498/90‑1 (MQ=255) gCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATgtgt < 1:236602/44‑1 (MQ=255) gCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATgtgt > 2:236602/1‑44 (MQ=255) | ACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGT > NZ_CP009273/4153744‑4153899 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |