| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,210,075 | T→C | F518L (TTT→CTT) | aceK → | bifunctional isocitrate dehydrogenase kinase/phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,210,075 | 0 | T | C | 100.0% | 104.5 / NA | 29 | F518L (TTT→CTT) | aceK | bifunctional isocitrate dehydrogenase kinase/phosphatase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/19); total (10/19) | |||||||||||
GACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGTTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCG > NZ_CP009273/4209988‑4210160 | gACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGctt > 2:38890/1‑90 (MQ=255)cccctCCCGCCGCCGCGCCCCCCGGAAGTTTACCTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGctt < 1:375015/86‑1 (MQ=255) cgccgccgcGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACt < 1:133014/90‑1 (MQ=255) cgccgccgcGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACt < 2:311141/90‑1 (MQ=255) tATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGcc < 1:156635/90‑1 (MQ=255) cGGAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGAcc < 2:268200/90‑1 (MQ=255) cGGAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGAcc < 1:513570/90‑1 (MQ=255) gAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCcg < 2:89864/90‑1 (MQ=255) gACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCGt > 2:602039/1‑90 (MQ=255) cGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACt < 2:176235/68‑1 (MQ=255) cGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACt > 1:176235/1‑68 (MQ=255) cGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGAcc < 1:519735/83‑1 (MQ=255) cGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGAcc > 2:519735/1‑83 (MQ=255) cGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCGTAt > 2:518112/1‑90 (MQ=255) ccAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGc < 1:452640/90‑1 (MQ=255) gCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGt > 2:399619/1‑90 (MQ=255) tACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATg < 1:62846/90‑1 (MQ=255) tACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATg < 2:421046/90‑1 (MQ=255) gCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGc < 2:267555/72‑1 (MQ=255) gCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGc > 1:267555/1‑72 (MQ=255) cGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGc < 2:58811/90‑1 (MQ=255) cGTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGc < 2:182325/90‑1 (MQ=255) gTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGcc > 1:116027/1‑90 (MQ=255) gTCTCGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGcc > 2:603171/1‑90 (MQ=255) ctcGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGAcc > 1:14414/1‑53 (MQ=255) ctcGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGAcc < 2:14414/53‑1 (MQ=255) tcGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGc < 2:289894/86‑1 (MQ=255) tcGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGc > 1:289894/1‑86 (MQ=255) cGCCGGGCGATGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCATATTGGTCCGCTGTTTGAAGAGATGCACGCCGAcc < 1:496315/90‑1 (MQ=255) tGTTTTCCCGGAAGAGCTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCgcgc < 1:602039/90‑1 (MQ=255) agagCTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGgcg < 1:586724/90‑1 (MQ=255) | GACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGTTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCG > NZ_CP009273/4209988‑4210160 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |