Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,263,211	T→C	K197E (AAA→GAA)	uvrA ←	excinuclease ABC subunit UvrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,263,211	0	T	C	100.0%	137.8 / NA	37	K197E (AAA→GAA)	uvrA	excinuclease ABC subunit UvrA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/15);  total (22/15)

GCGGTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTTCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAGGT  >  NZ_CP009273/4263129‑4263297                                                                                   |                                                                                       gcgGTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGc                                                                                 <  1:29137/90‑1 (MQ=255) gcgGTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGc                                                                                 <  1:217804/90‑1 (MQ=255)   ggTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAg                                                                               >  2:183445/1‑90 (MQ=255)      ttCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTc                                                                            >  2:471369/1‑90 (MQ=255)                 cGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCg                                                                 <  2:415863/90‑1 (MQ=255)                          gTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGaaa                                                        <  2:97781/90‑1 (MQ=255)                                     tCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACt                                             <  1:482209/90‑1 (MQ=255)                                       gTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTc                                           >  1:540995/1‑90 (MQ=255)                                       gTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTc                                           >  2:599353/1‑90 (MQ=255)                                       gTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTc                                           >  1:136992/1‑90 (MQ=255)                                       gTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTc                                           >  1:210170/1‑90 (MQ=255)                                        tCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCg                                          <  1:291596/90‑1 (MQ=255)                                         cACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGc                                         <  2:210499/90‑1 (MQ=255)                                            gCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCAt                                      <  1:177577/90‑1 (MQ=255)                                               ccTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCaa                                   >  1:21226/1‑90 (MQ=255)                                                 ttGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATa                                 >  2:488220/1‑90 (MQ=255)                                                       cGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCa                           >  1:419880/1‑90 (MQ=255)                                                        gATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCAc                          <  2:141391/90‑1 (MQ=255)                                                        gATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCAc                          <  1:471369/90‑1 (MQ=255)                                                         aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCAc                          >  2:201679/1‑89 (MQ=255)                                                         aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCAc                          <  1:201679/89‑1 (MQ=255)                                                         aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACg                         >  1:128722/1‑90 (MQ=255)                                                         aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACg                         >  1:608488/1‑90 (MQ=255)                                                         aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACg                         <  2:157443/90‑1 (MQ=255)                                                         aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACg                         >  2:168501/1‑90 (MQ=255)                                                         aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACg                         >  1:389298/1‑90 (MQ=255)                                                         aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACg                         <  1:488220/90‑1 (MQ=255)                                                         aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACg                         >  2:227088/1‑90 (MQ=255)                                                              ccaccaCTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGt                    <  1:64697/90‑1 (MQ=255)                                                                accacTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAg                  >  2:573494/1‑90 (MQ=255)                                                                   acTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGccc               >  1:427170/1‑90 (MQ=255)                                                                    cTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCt              >  2:77967/1‑90 (MQ=255)                                                                             tATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCa     <  2:338848/90‑1 (MQ=255)                                                                              aTGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAg    >  1:183180/1‑90 (MQ=255)                                                                              aTGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAg    >  1:428816/1‑90 (MQ=255)                                                                              aTGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAg    >  1:21436/1‑90 (MQ=255)                                                                                gTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAGGt  >  2:266/1‑90 (MQ=255)                                                                                   |                                                                                       GCGGTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTTCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAGGT  >  NZ_CP009273/4263129‑4263297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑