Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,338,978	A→C	intergenic (‑417/+154)	dcuB ← / ← dcuR	anaerobic C4‑dicarboxylate transporter DcuB/two‑component system response regulator DcuR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,338,978	0	A	C	100.0%	24.5 / NA	8	intergenic (‑417/+154)	dcuB/dcuR	anaerobic C4‑dicarboxylate transporter DcuB/two‑component system response regulator DcuR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (5/3);  total (5/3)

CTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTT  >  NZ_CP009273/4338892‑4339050                                                                                       |                                                                         cTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAccac                                                                       <  1:166769/90‑1 (MQ=255) cTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAccac                                                                       <  2:351778/90‑1 (MQ=255)    aTCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAACCACCGt                                                                    <  1:549506/90‑1 (MQ=255)      cTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAACCACCGTTc                                                                  <  1:557021/90‑1 (MQ=255)                                       gggTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAACCACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATgg                                 >  2:111496/1‑90 (MQ=255)                                        ggTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAACCACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGa                                >  2:472365/1‑90 (MQ=255)                                        ggTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAACCACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGa                                >  2:79973/1‑90 (MQ=255)                                                     ttaattaaCTTTTGTTAGCGTTTTGAAATTAAAACCACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGa                                >  1:172299/1‑77 (MQ=255)                                                     ttaattaaCTTTTGTTAGCGTTTTGAAATTAAAACCACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGa                                <  2:172299/77‑1 (MQ=255)                                                                      gCGTTTTGAAATTAAAACCACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGtt  >  1:473185/1‑90 (MQ=255)                                                                                       |                                                                         CTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTT  >  NZ_CP009273/4338892‑4339050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑