Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,459,020	T→C	Y527H (TAC→CAC)	mgtA →	magnesium‑translocating P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,459,020	0	T	C	100.0%	132.1 / NA	36	Y527H (TAC→CAC)	mgtA	magnesium‑translocating P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/17);  total (19/17)

TTAAGCGGGTTACTGATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAGAGACAACCGCTCC  >  NZ_CP009273/4458934‑4459099                                                                                       |                                                                                ttAAGCGGGTTACTGATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCAcc                                                                              >  1:465892/1‑90 (MQ=255)   aaGCGGGTTACTGATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAg                                                                            >  2:284672/1‑90 (MQ=255)   aaGCGGGTTACTGATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAg                                                                            >  1:150814/1‑90 (MQ=255)   aaGCGGGTTACTGATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAg                                                                            >  2:176787/1‑90 (MQ=255)   aaGCGGGTTACTGATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAg                                                                            >  1:399754/1‑90 (MQ=255)        ggTTACTGATACGCTGAATCGTCAGGGGCTGCGGGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAgcggg                                                                        <  1:377796/89‑1 (MQ=255)        ggTTACTGATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAgcggg                                                                        >  2:377796/1‑89 (MQ=255)            aCTGATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGAt                                                                   <  1:201903/90‑1 (MQ=255)               gATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGaa                                                                >  2:190390/1‑90 (MQ=255)                 tACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATc                                                              <  2:399754/90‑1 (MQ=255)                     cTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGAt                                                                   >  2:267741/1‑81 (MQ=255)                     cTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGAt                                                                   <  1:267741/81‑1 (MQ=255)                      tGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGAcc                                                         >  2:267265/1‑90 (MQ=255)                            gTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATcc                                                   >  1:157984/1‑90 (MQ=255)                             tCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCt                                                  <  1:506070/90‑1 (MQ=255)                             tCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCt                                                  <  1:176787/90‑1 (MQ=255)                              cAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTc                                                 >  1:115896/1‑90 (MQ=255)                                 gggCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCTGCGGGCGGATGAATCCGACCTGATCCTCGaa                                              <  2:465892/90‑1 (MQ=255)                                      gcgcGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAgcgggcgggt                                                                   >  1:406221/1‑62 (MQ=255)                                      gcgcGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGAt                                                                   <  2:406221/64‑1 (MQ=255)                                      gcgcGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGa                                               <  1:248115/84‑1 (MQ=255)                                      gcgcGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGa                                               >  2:248115/1‑84 (MQ=255)                                      gcgcGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAg                                             >  1:358248/1‑86 (MQ=255)                                      gcgcGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAg                                             <  2:358248/86‑1 (MQ=255)                                      gcgcGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGata                                         >  1:396540/1‑90 (MQ=255)                                            ggTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGc                                   >  2:544508/1‑90 (MQ=255)                                            ggTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGc                                   >  2:237077/1‑90 (MQ=255)                                            ggTTGCGGTGGCGACGAAATACCTGCCAGCGCGCGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGc                                   <  2:466248/90‑1 (MQ=255)                                                     ggCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGa                          >  1:212716/1‑90 (MQ=255)                                                     ggCGACGAAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGa                          >  2:483057/1‑90 (MQ=255)                                                        gacgaAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATcc                       <  1:575013/90‑1 (MQ=255)                                                        gacgaAATACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATcc                       <  1:111764/90‑1 (MQ=255)                                                             aaTACCTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCaa                  <  2:500138/90‑1 (MQ=255)                                                                  cTGCCAGCGCGTGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAagag             <  1:364749/90‑1 (MQ=255)                                                                             tGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAGAGACAACCGCTcc  <  2:186168/90‑1 (MQ=255)                                                                             tGAAGGAGATCACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAGAGACAACCGCTcc  <  2:115896/90‑1 (MQ=255)                                                                                       |                                                                                TTAAGCGGGTTACTGATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAGAGACAACCGCTCC  >  NZ_CP009273/4458934‑4459099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑