| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,903 | T→G | D27E (GAT→GAG) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,903 | 0 | T | G | 100.0% | 60.3 / NA | 20 | D27E (GAT→GAG) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (13/7); total (13/7) | |||||||||||
TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCA > NZ_CP009273/49820‑49987 | tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGcc < 2:337335/90‑1 (MQ=255) tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTa > 1:318758/1‑90 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGGGGAACCTGCCTGCCGAGCTCGCCTGGTTTaaa > 1:279852/1‑90 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCaa < 2:589568/90‑1 (MQ=255) aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAac < 2:465046/90‑1 (MQ=255) cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCt < 2:197268/90‑1 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCtt > 2:162978/1‑90 (MQ=255) ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTtaaat < 2:599540/90‑1 (MQ=255) gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTg > 2:139532/1‑90 (MQ=255) aTCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGcc > 1:572492/1‑48 (MQ=255) aTCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGcc < 2:572492/48‑1 (MQ=255) tCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgcc > 2:597710/1‑90 (MQ=255) cGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg > 1:282402/1‑90 (MQ=255) aCGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTggg > 1:389162/1‑90 (MQ=255) aTGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca > 2:17292/1‑90 (MQ=255) aTGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca > 1:9905/1‑90 (MQ=255) aTGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca > 2:342733/1‑90 (MQ=255) ggAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc < 2:287378/90‑1 (MQ=255) gccGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCa > 2:79786/1‑90 (MQ=255) gccGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCa > 2:252502/1‑90 (MQ=255) | TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCA > NZ_CP009273/49820‑49987 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |