Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	650,302	A→G	F375L (TTT→CTT)	dcuC ←	anaerobic C4‑dicarboxylate transporter DcuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	650,302	0	A	G	100.0%	42.2 / NA	13	F375L (TTT→CTT)	dcuC	anaerobic C4‑dicarboxylate transporter DcuC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/7);  total (6/7)

GGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCGGGATCATCTCAACAAACGCATAAAACGGCGCATTGCCTGAACCGGTCGTGACTGCCGCCAGCATTGTCAGAATCACCAATACCAGCATC  >  NZ_CP009273/650218‑650375                                                                                     |                                                                          ggTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCGGGATCATCTCAACAAGCGCAt                                                                      <  1:183591/90‑1 (MQ=255)     tGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCGGGATCATCTCAACAAGCGCATaaaa                                                                  >  1:161659/1‑90 (MQ=255)           cTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCGGGATCATCTCAACAAGCGCATAAAACGgcgc                                                            >  1:448790/1‑90 (MQ=255)                                aTACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCGGGATCATCTCAACAAGCGCATAAAACGGCGCAtt                                                         <  1:374733/72‑1 (MQ=255)                                aTACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCGGGATCATCTCAACAAGCGCATAAAACGGCGCAtt                                                         >  2:374733/1‑72 (MQ=255)                                       gggTTAATGCCGGAAGAGTGCGCCAGTTTCGGGATCATCTCAACAAGCGCATAAAACGGCGCATTGCCTGAACCGGTCGTGACTgccgcc                                >  2:188296/1‑90 (MQ=255)                                                 cGGAAGAGAGCGCCAGTTTCGGGATCATCTCAACAAGCGCATAAAACGGCGCATTGCCTGAACCGGTCGTGACTGCCGCCAGCATTGTCa                      >  1:511681/1‑90 (MQ=255)                                                     agagTGCGCCAGTTTCGGGATCATCTCAACAAGCGCATAAAACGGCGCATTGCCTGAACCGGTCGTGACTGCCGCCAGCATTGTCAGAAt                  <  1:253178/90‑1 (MQ=255)                                                               tgTTTCGGGATCATCTCAACAAGCGCATAAAACGGCGCATTGCCTGAACCGGTCGTGACTGCCGCCAGCATTGTCAGAATCACCAATAcc        <  2:511681/89‑1 (MQ=255)                                                               aGTTTCGAGATCATCTCAACAAGCGCATAAAACGGCGCATTGCCTGAACCGGTCGTGACTGCCGCCAGCATTGTCAGAATCACCAATAcc        <  2:23288/90‑1 (MQ=255)                                                                     gggATCATCTCAACAAGCGCATAAAACGGCGCATTGCCTGAACCGGTCGTGACTGCCGCCAGCATTGTCAGAATCACCAATACCAGCATc  <  2:148332/90‑1 (MQ=255)                                                                          catcTCAACAAGCGCATAAAACGGCGCATTGCCTGAACCGGTCGTGACTGCCGCCAGCATTGt                        >  1:609962/1‑63 (MQ=255)                                                                          catcTCAACAAGCGCATAAAACGGCGCATTGCCTGAACCGGTCGTGACTGCCGCCAGCATTGt                        <  2:609962/63‑1 (MQ=255)                                                                                     |                                                                          GGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCGGGATCATCTCAACAAACGCATAAAACGGCGCATTGCCTGAACCGGTCGTGACTGCCGCCAGCATTGTCAGAATCACCAATACCAGCATC  >  NZ_CP009273/650218‑650375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑