Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	802,549	T→G	T63T (ACA→ACC)	ybhC ←	putative acyl‑CoA thioester hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	802,549	0	T	G	100.0%	19.4 / NA	7	T63T (ACA→ACC)	ybhC	putative acyl‑CoA thioester hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (2/5);  total (2/5)

CCTTGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGG  >  NZ_CP009273/802466‑802622                                                                                    |                                                                          ccTTGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCTCCTGGGGTCAgg                                                                     <  1:165477/90‑1 (MQ=255)         gCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGc                                                                >  1:37052/1‑87 (MQ=255)         gCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGc                                                                <  2:37052/87‑1 (MQ=255)           ccgccgGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAAt                                                           <  1:391231/90‑1 (MQ=255)             gccgGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAATAg                                                         <  2:580558/90‑1 (MQ=255)                                      gcgcAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGctt                                >  2:138536/1‑90 (MQ=255)                                                                    aCGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAgg  <  1:98202/90‑1 (MQ=255)                                                                                    |                                                                          CCTTGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGG  >  NZ_CP009273/802466‑802622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑