Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	876,371	T→C	D63D (GAT→GAC)	dacC →	serine‑type D‑Ala‑D‑Ala carboxypeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	876,371	0	T	C	100.0%	40.6 / NA	12	D63D (GAT→GAC)	dacC	serine‑type D‑Ala‑D‑Ala carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/7);  total (5/7)

GCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATA  >  NZ_CP009273/876297‑876450                                                                           |                                                                                gcgcGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGACCCCGCGAGCCTGACt                                                                  >  1:527790/1‑90 (MQ=255)  cgcgTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGACCCCGCGAGCCTGACTa                                                                 <  1:477385/90‑1 (MQ=255)   gcgTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGACCCCGCGAGCCTGACTaa                                                                >  1:245759/1‑90 (MQ=255)           ggATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGACCCCGCGAGCCTGACTAAAATCATGa                                                        <  2:129812/90‑1 (MQ=255)            gATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGACCCCGCGAGCCTGACTAAAATCATGAc                                                       >  2:169217/1‑90 (MQ=255)             aTTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGACCCCGCGAGCCTGACTAAAATCATGAcc                                                      <  1:260946/90‑1 (MQ=255)             aTTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGACCCCGCGAGCCTGACTAAAATCATGAcc                                                      <  1:497446/90‑1 (MQ=255)                          aCGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGACCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTg                                         >  1:74760/1‑90 (MQ=255)                                gCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGACCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAgg                                   <  1:556688/90‑1 (MQ=255)                                gCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGACCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAgg                                   >  2:257486/1‑90 (MQ=255)                                gCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGACCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAgg                                   <  2:611310/90‑1 (MQ=255)                                                                 agaAACTGGACCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGata  <  2:276408/90‑1 (MQ=255)                                                                           |                                                                                GCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATA  >  NZ_CP009273/876297‑876450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑