| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 50,280 | T→C | F153S (TTT→TCT) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 50,280 | 0 | T | C | 100.0% | 67.5 / NA | 19 | F153S (TTT→TCT) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/12); total (7/12) | |||||||||||
TTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCC > NZ_CP009273/50194‑50364 | tttCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTga > 1:152219/1‑90 (MQ=255)tttCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTga < 1:41085/90‑1 (MQ=255)tttCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTga < 1:487236/90‑1 (MQ=255) ttCCCGGATTACAAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTgag < 1:561990/90‑1 (MQ=255) ggATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCt > 2:425289/1‑90 (MQ=255) tACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAg < 2:210559/90‑1 (MQ=255) cGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAgcg > 2:168949/1‑90 (MQ=255) gACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAAttt < 1:79786/90‑1 (MQ=255) gACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAAttt < 2:13291/90‑1 (MQ=255) gggAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGGAATTTTGta > 1:76242/1‑90 (MQ=255) aTCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGa < 1:75110/90‑1 (MQ=255) aaTTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGGAATTTTTTATAGAATTTACGGCTAgcg > 1:160473/1‑90 (MQ=255) cgcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTc < 1:500032/90‑1 (MQ=255) cgcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTc < 2:568451/90‑1 (MQ=255) gAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGt < 2:424934/90‑1 (MQ=255) cacaGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTTTATAAAATTTACGGCTAGCCCCCGATGCGGCGCCGGGCCCGTCTTTTcc > 2:601207/1‑90 (MQ=255) aGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGc < 1:202008/90‑1 (MQ=255) aGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGc < 2:76242/90‑1 (MQ=255) ttGCTCTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAATTTACGGCTAGCGCCCGATGCGACGCCGGGCGCGTCTTTTCCGGCCCTcc > 2:382016/1‑90 (MQ=255) | TTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCC > NZ_CP009273/50194‑50364 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |