Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,065,426	T→G	T129P (ACC→CCC)	rutF ←	pyrimidine utilization flavin reductase protein F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,065,426	0	T	G	100.0%	37.1 / NA	11	T129P (ACC→CCC)	rutF	pyrimidine utilization flavin reductase protein F
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/4);  total (7/4)

TGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAG  >  NZ_CP009273/1065341‑1065483                                                                                      |                                                          tGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCg                                                       >  2:76694/1‑90 (MQ=255)       cTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTc                                                 <  2:20939/90‑1 (MQ=255)             tCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCaccacc                                           <  1:130205/90‑1 (MQ=255)                              gTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTc                          >  2:243594/1‑90 (MQ=255)                                          gtgACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTcac                                               >  1:537044/1‑57 (MQ=255)                                          gtgACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTcac                                               <  2:537044/57‑1 (MQ=255)                                             aCGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGc           >  1:305789/1‑90 (MQ=255)                                                    aTCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTcc    >  1:153815/1‑90 (MQ=255)                                                      cGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAg  >  2:315922/1‑90 (MQ=255)                                                                                 gTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCa               <  1:50678/50‑1 (MQ=255)                                                                                 gTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCa               >  2:50678/1‑50 (MQ=255)                                                                                      |                                                          TGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAG  >  NZ_CP009273/1065341‑1065483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑