Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,174,948	T→C	H300H (CAT→CAC)	nagK →	N‑acetylglucosamine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,174,948	0	T	C	100.0%	36.5 / NA	11	H300H (CAT→CAC)	nagK	N‑acetylglucosamine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/4);  total (7/4)

TGTAGCTCGTGTTCCGCGCATTGAACGCGCGCGCCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATA  >  NZ_CP009273/1174873‑1175021                                                                            |                                                                          tGTAGCTCGTGTTCCGCGCATTGAACGCGCGCGCCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACACCTAACCGATTAaac                                                             <  1:101779/90‑1 (MQ=255)         gtgtTCCGCGCATTGAACGCGCGCGCCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACACCTAACCGATTAAACAACAGAgg                                                     >  1:365582/1‑90 (MQ=255)            ttCCGCGCATTGAACGCGCGCGCCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACACCTAACCGATTAAACAACAGAGGTTg                                                  >  2:300079/1‑90 (MQ=255)                  gcATTGAACGCGCGCGCCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACACCTAACCGATTAAACAACAGAGGTTGCTATGc                                            <  2:114428/90‑1 (MQ=255)                                 gcCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACACCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTc                             >  2:590653/1‑90 (MQ=255)                                            gCGGGAGGAATGCGTGGTGCGGCCTTCCTACACCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGt                  >  1:300332/1‑90 (MQ=255)                                                         gtggtgCGGCCTTCCTACACCTAACCGATTAAACAACagag                                                      <  1:222999/41‑1 (MQ=255)                                                         gtggtgCGGCCTTCCTACACCTAACCGATTAAACAACagag                                                      >  2:222999/1‑41 (MQ=255)                                                         gtggtgCGGCCTTCCTACACCTAACCGATTAAACAACaga                                                       <  1:377388/40‑1 (MQ=255)                                                         gtggtgCGGCCTTCCTACACCTAACCGATTAAACAACaga                                                       >  2:377388/1‑40 (MQ=255)                                                            gtgCGGCCTTCCTACACCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATa  >  2:98567/1‑90 (MQ=255)                                                                            |                                                                          TGTAGCTCGTGTTCCGCGCATTGAACGCGCGCGCCACGGTGATGCGGGAGGAATGCGTGGTGCGGCCTTCCTACATCTAACCGATTAAACAACAGAGGTTGCTATGCTGTCGCGTCGGGGTCATCGGTTAAGTCGTTTTCGTAAAAATA  >  NZ_CP009273/1174873‑1175021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑