Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,443,919	T→A	D563V (GAT→GTT)	tynA ←	primary‑amine oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,443,919	0	T	A	100.0%	68.1 / NA	18	D563V (GAT→GTT)	tynA	primary‑amine oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (8/10);  total (8/10)

GTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGTCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCG  >  NZ_CP009273/1443842‑1443989                                                                              |                                                                       gTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGTCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGAACGAGGCGGAAAt                                                            >  1:424015/1‑90 (MQ=255) gTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGTCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGAACGAGGCGGAAAt                                                            >  1:428630/1‑90 (MQ=255) gTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGTCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGAACGAGGCGGAAAt                                                            >  2:57436/1‑90 (MQ=255) gTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGTCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGAACGAGGCGGAAAt                                                            >  2:526913/1‑90 (MQ=255) gTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGTCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGAACGAGGCGGAAAt                                                            >  1:277476/1‑90 (MQ=255)        aCCGGCAGTATTCGGTTTTACCACTGGGTCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGAACGAGGCGGAAATTATAAat                                                     <  2:216740/90‑1 (MQ=255)               gTATTCGGTTTTACCACTGGGTCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGAACGAGGCGGAAATTATAAATATGTTgg                                              <  2:587883/90‑1 (MQ=255)                 aTTCGGTTTTACCACTGGGTCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGAACGAGGCGGAAATTATAAATATGTTGgtg                                            <  2:28416/90‑1 (MQ=255)                          tACCACTGGGTCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGAACGAGGCGGAAATTATAAATATGTTGGTGTGTAGTAcc                                   <  2:424015/90‑1 (MQ=255)                               cTGGGTCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGAACGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGa                              <  2:398544/90‑1 (MQ=255)                                   gTCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGAACGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATAtt                          >  1:97930/1‑90 (MQ=255)                                     ccATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGAACGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATtgt                        <  2:26397/90‑1 (MQ=255)                                     ccATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGAACGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATtgt                        <  1:526913/90‑1 (MQ=255)                                       aTCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGAACGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGa                      <  2:277476/90‑1 (MQ=255)                                                     gTTATTCTCGCCATCTACATCCAGAACGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGcc        >  2:252295/1‑90 (MQ=255)                                                           ctcGCCATCTACATCCAGAACGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAgcg  <  1:612772/90‑1 (MQ=255)                                                               ccATCTACATCCAGAACGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCAc                                >  1:591092/1‑56 (MQ=255)                                                               ccATCTACATCCAGAACGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCAc                                <  2:591092/56‑1 (MQ=255)                                                                              |                                                                       GTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGTCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCG  >  NZ_CP009273/1443842‑1443989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑