Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,454,774	A→G	D16G (GAT→GGT)	paaI →	hydroxyphenylacetyl‑CoA thioesterase PaaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,454,774	0	A	G	100.0%	43.9 / NA	13	D16G (GAT→GGT)	paaI	hydroxyphenylacetyl‑CoA thioesterase PaaI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/7);  total (6/7)

TGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACC  >  NZ_CP009273/1454688‑1454844                                                                                       |                                                                       tGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGc                                                                     >  2:322416/1‑90 (MQ=255)                cAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCt                                                      <  2:59019/90‑1 (MQ=255)                                     ggTTATGGGTCATAAGGCCTGGGAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAAt                                 <  1:470387/90‑1 (MQ=255)                                     ggTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAAt                                 <  1:60559/90‑1 (MQ=255)                                     ggTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCCCCAAAGCGCTTGGCATCGACATTATCTCAAt                                 >  2:212237/1‑90 (MQ=255)                                            aGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGaa                          <  1:100341/90‑1 (MQ=255)                                              tCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAgg                        >  2:56955/1‑90 (MQ=255)                                                aTAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCtt                                                     >  1:462903/1‑59 (MQ=255)                                                aTAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCtt                                                     <  2:462903/59‑1 (MQ=255)                                                       cTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGt               >  2:143389/1‑90 (MQ=255)                                                       cTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGt               >  2:500755/1‑90 (MQ=255)                                                                    cATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGAcc  <  1:212237/90‑1 (MQ=255)                                                                                    aCGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGc                  <  2:39301/58‑1 (MQ=255)                                                                                       |                                                                       TGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACC  >  NZ_CP009273/1454688‑1454844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑