Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,003,480	T→C	F60S (TTC→TCC)	yedK →	SOS response‑associated peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,003,480	0	T	C	100.0%	18.7 / NA	7	F60S (TTC→TCC)	yedK	SOS response‑associated peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/3);  total (4/3)

ACCCATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAAC  >  NZ_CP009273/2003394‑2003566                                                                                       |                                                                                       aCCCATTGGCAGATACAACGTCGCGTCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTg                                                                                     <  1:393795/90‑1 (MQ=255)    cATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTgggg                                                                                  >  2:599939/1‑90 (MQ=255)                                           aCTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTaa                                           >  1:446325/1‑90 (MQ=255)                                              gCTCAGTGAACGTGATGAACACCTCCATCTGGATCCGGTTTCTTGGGGATATGCTCCCGGATGGTGGGATAACCCGCCGCTGATTAACGc                                        <  1:499276/90‑1 (MQ=255)                                                tCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGccc                                      <  2:446325/90‑1 (MQ=255)                                                   gTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCcgcg                                   >  1:301813/1‑90 (MQ=255)                                                           gatgaACACCTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACt                           >  2:154164/1‑90 (MQ=255)                                                                                    tttcCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAAc  <  2:420907/90‑1 (MQ=255)                                                                                       |                                                                                       ACCCATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAAC  >  NZ_CP009273/2003394‑2003566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑