Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,738,4480AC54.2% 8.5 / 23.0 24V84G (GTG→GGG) trmDtRNA (guanosine(37)‑N1)‑methyltransferase TrmD
Reads supporting (aligned to +/- strand):  ref base A (6/5);  new base C (0/13);  total (6/18)
Fisher's exact test for biased strand distribution p-value = 3.43e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCC  >  NZ_CP009273/2738359‑2738533
                                                                                         |                                                                                     
gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                       <  1:380963/90‑1 (MQ=255)
  ccGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCAcc                                                                                     >  1:274050/1‑90 (MQ=255)
                  aTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                       <  1:1099255/72‑1 (MQ=255)
                  aTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                       >  2:1099255/1‑72 (MQ=255)
                     tttGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACccgccg                                                                  <  2:406993/90‑1 (MQ=255)
                       tGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCcgcg                                                                >  2:491053/1‑90 (MQ=255)
                                          tGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATgg                                             <  2:1060750/90‑1 (MQ=255)
                                                   cTTGATCAAGCTTGCGTCCCTGTGGTGACAGAAAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCATCCCGCa                                    <  1:464508/90‑1 (MQ=255)
                                                     tGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAg                                  <  2:1046633/90‑1 (MQ=255)
                                                     tGATCAAGCTTGCGCCCCTGTGGTGACAGAAAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAg                                  <  1:720545/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  1:237718/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  2:917901/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  2:455235/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGAAAGAAAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  1:102443/90‑1 (MQ=255)
                                                             cTTGCGCCCCGGTGGTGACAGATAAATCCCCTTTGCCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  1:491053/90‑1 (MQ=255)
                                                              ttGCGTCCTTGTGGTGACAGAAAAACCCCCTTTGCCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAcc                         <  1:718817/90‑1 (MQ=255)
                                                              ttGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAcc                         <  2:614854/90‑1 (MQ=255)
                                                                gCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcat                       >  1:688436/1‑90 (MQ=255)
                                                                   tCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcatcat                    <  2:467541/90‑1 (MQ=255)
                                                                   tCCCTGTGGTGACAAAAAAATCCCCTTTCCGCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcatcat                    <  2:763932/90‑1 (MQ=255)
                                                                     cgtgtgTTGAAAGATAAACCCCCTTTGCGCCTTCTCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTa                  <  2:674748/88‑1 (MQ=255)
                                                                      cTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATgg                                             >  1:75269/1‑62 (MQ=255)
                                                                      cTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATgg                                             <  2:75269/62‑1 (MQ=255)
                                                                          ggtgACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATc             >  2:88199/1‑90 (MQ=255)
                                                                             gACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATcccc          >  2:768420/1‑90 (MQ=255)
                                                                               cAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCgg        <  2:274050/90‑1 (MQ=255)
                                                                                     aaTCCCCTTTGCCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgcc  <  2:359377/90‑1 (MQ=255)
                                                                                         |                                                                                     
GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCC  >  NZ_CP009273/2738359‑2738533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: