| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,291,076 | 0 | A | C | 52.4% | 11.4 / 26.3 | 21 | V141G (GTG→GGG) | yraQ | permease |
| Reads supporting (aligned to +/- strand): ref base A (8/2); new base C (0/11); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.21e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAG > NZ_CP009273/3290988‑3291158 | ccAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGacac > 1:890658/1‑90 (MQ=255) gAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTg > 2:392787/1‑90 (MQ=255) aTAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTccc > 2:25172/1‑90 (MQ=255) aGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGAcc > 2:354660/1‑90 (MQ=255) gTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGa > 1:349930/1‑90 (MQ=255) cggcGGGTTTAACCCGGGGTCGCCCATCCATAATGACGGCGCACCGCCCATCGCCCCCTGTTGGCGACGCTTTCCCGCCGCGACCGGAGc < 2:901065/87‑1 (MQ=255) cGCCGGGTTTAACACCGGATTGCCCACCCAGAACGCCAGCGCCCCCCCCACCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGc < 1:358238/90‑1 (MQ=255) gTTTAACCCCGGATTGCCCATCCAGAAGGCCAGCGCCCCGCCCATCGACCCCTGTTGGCGACGCATGCCCGCCGCGACCGGAGCCGcaca < 2:590151/90‑1 (MQ=255) gTTAAACCCCGGATTGCCCACCCAGAATGCCAGCGCCCCGCCCACCGCCCCCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGcaca < 1:666635/90‑1 (MQ=255) ttAACCCCGGATTGCCCATCCAAAATGCCAGCGCACCGCCCACCGACCCCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCAcagc < 2:147257/90‑1 (MQ=255) ttAACCCCGGATTCCCCACCAAGAATGCCAGCCCCCCGCCCATCGACCCCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCAcagc < 2:737671/90‑1 (MQ=255) gagacagCGGATTCCCCACCCAGAACGCCCGCGCCCCCCCCATCGACCCCTGTTGGCGACGCATCCCCGCCGCGACCGGAGCCGCAcagc < 2:716173/87‑1 (MQ=255) ccGGATTGCCCACCCAAAATGCCAGCGCCCCGCCCATCGACCCCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTac < 2:786224/90‑1 (MQ=255) tCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCg > 1:848924/1‑90 (MQ=255) ccAGAATGCCAGCCCCCCCCCCATCGCCCCCTTTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCgg < 2:89375/90‑1 (MQ=255) aacgCCAGCGCCCCCCCCATCGACCCCTGTGGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAAc < 1:93681/87‑1 (MQ=255) gcACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGc > 1:919338/1‑90 (MQ=255) ccccGCCCATCGACCCCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCg < 2:408475/88‑1 (MQ=255) ccccGCCCACCGACCCCTGTGGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCg < 2:919338/88‑1 (MQ=255) cACCGCCCATCGCCACCTGTTGGCGACGCTTTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCg < 2:123221/90‑1 (MQ=255) cGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTc > 1:1121868/1‑90 (MQ=255) cATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCcag > 1:942213/1‑90 (MQ=255) | CCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAG > NZ_CP009273/3290988‑3291158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |