Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,910	T→A	W30R (TGG→AGG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,910	0	T	A	100.0%	119.6 / NA	33	W30R (TGG→AGG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (17/16);  total (17/16)

ATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49823‑49996                                                                                        |                                                                                       aTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAgg                                                                                      >  1:996214/1‑90 (MQ=255)     tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTa                                                                                  <  1:358025/90‑1 (MQ=255)       aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTaaa                                                                                >  1:299956/1‑90 (MQ=255)       aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACACCATGCCGTGGAACCTGCCTGCCGATTTCGCCAGGCTTaaa                                                                                >  2:660714/1‑90 (MQ=255)            gATTGCGGCGTAAGCGGTAGATCGCGTTATCGGCATGGAAACCGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCaa                                                                           <  1:872639/90‑1 (MQ=255)                 cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCt                                                                      <  1:947018/90‑1 (MQ=255)                  ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCGATGCCGTGGAACCTGCCTGCCGGTCTCGCCAGGTTTAAACGCAACACCtt                                                                     >  2:872639/1‑90 (MQ=255)                  ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCtt                                                                     >  1:1029842/1‑90 (MQ=255)                  ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCtt                                                                     >  2:111339/1‑90 (MQ=255)                  ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCtt                                                                     >  1:189991/1‑90 (MQ=255)                             tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCt                                                                      <  1:1101156/78‑1 (MQ=255)                             tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCt                                                                      >  2:1101156/1‑78 (MQ=255)                              aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGt                                                         <  2:739928/90‑1 (MQ=255)                              aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGt                                                         <  1:753540/90‑1 (MQ=255)                                   gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCaa                                                                           >  2:381551/1‑67 (MQ=255)                                   gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCaa                                                                           <  1:381551/67‑1 (MQ=255)                                    cgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTAt                                                   >  1:141076/1‑90 (MQ=255)                                    cgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTAt                                                   <  1:134371/90‑1 (MQ=255)                                      ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                                 <  2:1083274/90‑1 (MQ=255)                                       tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                                <  2:920136/90‑1 (MQ=255)                                                                tGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgt                       >  2:550785/1‑90 (MQ=255)                                                                       tgcctgccGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                >  2:1073673/1‑90 (MQ=255)                                                                       tgcctgccGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                >  2:1047885/1‑90 (MQ=255)                                                                       tgcctgccGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                >  2:828609/1‑90 (MQ=255)                                                                       tgcctgccGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                >  2:563173/1‑90 (MQ=255)                                                                            gccGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                                     >  1:52185/1‑64 (MQ=255)                                                                            gccGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                                     <  2:52185/64‑1 (MQ=255)                                                                            gccGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCa           >  1:555040/1‑90 (MQ=255)                                                                                aTCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc       <  1:1047885/90‑1 (MQ=255)                                                                                aTCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc       <  2:733859/90‑1 (MQ=255)                                                                                     gCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  2:388091/90‑1 (MQ=255)                                                                                     gCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  2:1011568/90‑1 (MQ=255)                                                                                     gCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  1:59156/90‑1 (MQ=255)                                                                                        |                                                                                       ATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49823‑49996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑