| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 353,601 | 0 | C | A | 56.7% | 4.7 / 32.1 | 30 | L267F (TTG→TTT) | cynR | transcriptional regulator CynR |
| Reads supporting (aligned to +/- strand): ref base C (10/3); new base A (0/17); total (10/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.52e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAA > NZ_CP009273/353523‑353687 | aacaacCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGt > 2:514924/1‑90 (MQ=255) cccccGCGTTTTTATCCACCCCCTTGT‑CAAAAACGCCCTC‑CGCGGCGGCTGTCTGCAAGATTTTTTCCCGCCGCAAAAAAACCGCCGTtc < 1:682420/87‑1 (MQ=255) cacccGCGTTTTTACCCAACCCCATGGGAAAAAATC‑CTTT‑CGGGGGGGCTTTCGGCCGGTTTTTTTCCCGCCGCAAAAAAACCGCCGTtc < 1:23364/87‑1 (MQ=255) cacccGCGCTTTTACCCACCGCCTTGTGAAAAAATG‑CCTT‑CGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTtc < 2:662704/87‑1 (MQ=21) cacccGCGCTTTTACCCACCGCCATGTGAAAAAATG‑CCTT‑CGCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTtc < 2:32330/87‑1 (MQ=21) cacccGCGCATTTCCCCAACCCCATGTGAAAAAATC‑CCTC‑CGGGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTtc < 2:536579/87‑1 (MQ=16) cacccGCGCATTTACCCACCCCCATGTGAAAAAATC‑CCTT‑CGGGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTtc < 2:281065/87‑1 (MQ=21) cgcgCTTTTACCCACCCCCATGGAAAAAAATC‑CCTC‑CGGGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGAAAAAAACCCGCCGTtctctc < 1:108208/90‑1 (MQ=255) gcgcATTTATCCAACGCCATGTGCAAAAATG‑CCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTcc > 2:628305/1‑90 (MQ=255) gcgcATTTATCCAACGCCATGTGCAAAAATG‑CCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTcc > 1:297003/1‑90 (MQ=255) tATCCAACGCCATGTGCAAAAATG‑CCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTg > 1:288609/1‑90 (MQ=255) aCGCCATGTGCAAAAATG‑CCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 2:684425/1‑90 (MQ=255) aCGCCATGTGCAAAAATG‑CCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 1:408244/1‑90 (MQ=255) aCGCCATGTGCAAAAATG‑CCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 2:451181/1‑90 (MQ=255) aCGCCATGTGCAAAAATG‑CCTT‑CGCAGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 1:808956/1‑90 (MQ=255) cccttgtgAAAAAATG‑CGTC‑CGCGGCGGCTGTCTGCCAGTTTTTTTTCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCa < 2:297003/86‑1 (MQ=255) aTGTGCAAAAATG‑CCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGcccgg > 1:330967/1‑86 (MQ=255) aaaaaaCG‑CCTC‑GGGGGCGGCTGTCTGCCGGTTTTTTTCCCGCCGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAAt < 1:574974/89‑1 (MQ=255) aaaaaTT‑CCTTCCGGGGGGGCTGTCGGCCAGTTTTTTTCCCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAAt < 2:288609/90‑1 (MQ=255) aaaaTG‑CCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAAAGaaaaaa > 2:374540/1‑87 (MQ=39) t‑CGGGGCGGCTGTCTGCCCTTTTTTTTCCCGCCCAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc < 2:666556/90‑1 (MQ=255) gacgggcggcTTCCTCCCAGTTTTTTTCCCCCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc < 2:527761/86‑1 (MQ=255) ccggggcggcTGTCTGCCACTTTTTTTCCCCCCGCAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc < 2:590147/86‑1 (MQ=255) ccgcgGGGGCTGTCTGCCACTTTTTTTCCCGCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc < 2:196576/89‑1 (MQ=255) gcTGTCGGCCAGTTTTTTTCCCCCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAtgt < 2:67443/90‑1 (MQ=255) tttttcCCGCCCGAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc < 2:394515/89‑1 (MQ=255) tttttcCCCCCGCAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc < 2:43887/89‑1 (MQ=255) tttttcCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc < 2:882148/89‑1 (MQ=255) ttttcCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgct < 2:869798/90‑1 (MQ=255) tttcCCCCCGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctg < 2:502482/90‑1 (MQ=255) ccacaaaaaAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTaa < 2:383530/87‑1 (MQ=255) cacaaaaaACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAc < 1:221962/88‑1 (MQ=255) caacaaAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACaa < 1:684425/90‑1 (MQ=255) | AACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAA > NZ_CP009273/353523‑353687 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |