| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 612,014 | 0 | A | G | 58.3% | 6.0 / 11.5 | 12 | D801G (GAT→GGT) | entF | enterobactin non‑ribosomal peptide synthetase EntF |
| Reads supporting (aligned to +/- strand): ref base A (0/5); new base G (7/0); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCC > NZ_CP009273/611929‑612103 | gtgCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGGtctc > 1:590078/1‑90 (MQ=255) aTGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCTGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACt < 2:752890/90‑1 (MQ=255) aTGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACt < 2:590078/90‑1 (MQ=255) aTACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGgcgc < 1:679606/90‑1 (MQ=255) cTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTTTCTTTTTCACTTGCATTTAACTGGCGCCgggggat > 2:337034/1‑86 (MQ=255) cTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTTTCTTTCTCCCTTGCATTCAACCTGCGCCCGGCtgt > 2:528531/1‑88 (MQ=255) gATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTTTCTCCCTCGGCTTCAACTGGCGGAGGGGGGTCTCGGGCGcccc > 1:292738/1‑90 (MQ=255) gATGCGATGAGGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTTTCTCCCTTGCATTCAACTGGCGCCGGGGGATTTCGGGCGcccc > 2:675293/1‑90 (MQ=255) gCATCCGGTGCCGCCGGGTGTGGCGGGGGGTTTCTATCTCACTTGCCTTTAACTGGCGCCGGGGTATATTTGAAGCCCCCATCTGACCGc > 2:252529/1‑90 (MQ=255) ccgccgGGTGTGGCGGGGGGTCTCTTTCTCCCTGGCATTTAACTGGCGCCGGGGTATTTCGGGCGCCCCCCCCTTACCGCCCGCCGCctt > 2:572813/1‑90 (MQ=255) gtgGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGAt < 2:372604/90‑1 (MQ=255) gtgGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGAt < 2:705000/90‑1 (MQ=255) aTCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGccc < 2:727864/90‑1 (MQ=255) | GTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCC > NZ_CP009273/611929‑612103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |