| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 782,311 | 0 | A | C | 53.3% | 14.7 / 20.7 | 15 | G247G (GGT→GGG) | gpmA | 2,3‑diphosphoglycerate‑dependent phosphoglycerate mutase |
| Reads supporting (aligned to +/- strand): ref base A (7/0); new base C (0/8); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCG > NZ_CP009273/782232‑782395 | cTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTg > 2:240936/1‑90 (MQ=255)cTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTg > 2:844839/1‑90 (MQ=255) acaaAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCgctgct > 1:819956/1‑90 (MQ=255) gagaaaGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCttt > 2:23683/4‑90 (MQ=255) aaaGCCGCCCCCTTTGCGGTCGGTTTTCTCTTTTAAAACGAATGACTTTTCCTTCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGc < 1:844839/90‑1 (MQ=255) ccccACTTGCGGTCGGCTTTCTCATTTAAAACGAATGACGTTTCCTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGAtc < 1:9693/87‑1 (MQ=255) aCTCCCTGGCAGTCGTTTTTCTCATTTAAAACGAATGACTTTTCCTTCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGAtc < 1:89209/90‑1 (MQ=255) aGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCAt < 2:151189/90‑1 (MQ=255) cGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTAc > 1:421770/1‑90 (MQ=255) ggCTTTCTCTTTTTAAACGAATGACTTTTTCTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTAcc < 1:23683/90‑1 (MQ=255) ggCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTAcc < 2:303960/90‑1 (MQ=255) tttAAACGAATGACTTTTCCTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGc < 1:269980/90‑1 (MQ=255) aaaCGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGtt > 2:373018/1‑90 (MQ=255) aaTGCCTTTTTCTTCTCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGc < 1:209733/90‑1 (MQ=255) gCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCtcg > 1:537188/1‑90 (MQ=255) | CTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCG > NZ_CP009273/782232‑782395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |