| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,906,007 | 0 | T | G | 68.4% | 5.4 / 13.3 | 19 | H276P (CAC→CCC) | htpX | protease HtpX |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (13/0); total (14/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.16e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.34e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTT > NZ_CP009273/1905919‑1906079 | gATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCgggtg < 1:804507/90‑1 (MQ=255) aCGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATg < 2:37632/90‑1 (MQ=255) tttACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGGGGGGCATGAACAAATCACTGAGc > 2:110270/1‑90 (MQ=255) cATATTACTTCAGGTATTCACCCGTACGCAGAGCTCCAATTCGTTTATCCAGCGGCGGGGGGGGCATGAACAACTCACTGAGCGGttttg > 2:9492/1‑88 (MQ=255) cTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTAcc < 1:176454/90‑1 (MQ=255) ggTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGGGGGGCATGAACAACTCCCTGAGCGATTTTGGCTTACCCTTaa > 2:664805/1‑90 (MQ=255) ggTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGGGGGGCATGAACAACTCACTGAGCGATTTCGACTTACCGTTaa > 2:55599/1‑90 (MQ=255) tCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCag < 1:303800/90‑1 (MQ=255) tCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGGGGGTCATGAACAACTCACTGAGCGATTTTGACTTACCGTTAATGCag > 2:243398/1‑90 (MQ=255) cGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGc < 2:231510/90‑1 (MQ=255) gTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGGGGGGGCTGAACAACTCACTGAGGGATTTCGACTTTCCGGTAATGGagaggggc > 1:102930/1‑86 (MQ=255) gTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGGGGGGGCTGAACAACCCACCTAGCGGTTTTTTCTTTCCCTTTATGCGGAGAGcc > 1:645773/1‑90 (MQ=255) gTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGGGGGGCATGAAAAACTCACTGAGCGATTTTGACTTACCCTTAATTCagagaggc > 2:817885/1‑88 (MQ=255) aCGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGGGGGGCCTGAACAAAACACCCAGCGATTTTTACTTACCCGTAATGCAGAGAGGcag > 1:566069/1‑89 (MQ=255) gagCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTa < 1:675124/65‑1 (MQ=255) gagCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTa > 2:675124/1‑65 (MQ=255) ttCAATTCGTTTATCCAGCGGCGGGGGGGGCATTAAAAAAACACTGAGCGATTTTTACTTTCCGTTAATGCAGAGAGGCATCATTCTggg > 1:865572/1‑89 (MQ=255) ttCAATTCGTTTATCCAGCGGCGGGGGGGGCATGAACAACTCACTGAGCGATTTTGACTTACCGTTAATGCAGAGAGCCAACATTCTggg > 2:634055/1‑89 (MQ=255) tCAATTCGTTTATCCAGCGGCGGGGGGGGGATGAAAAACTCACTGAGCGATTTTGACTTACCGTTAATGCAGAGAGCCATCATGCTgggt > 1:539272/1‑88 (MQ=255) gTTTATCCAGCGGCGGGGGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGcttctt > 2:31876/1‑90 (MQ=255) | GATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTT > NZ_CP009273/1905919‑1906079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |