| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,571 | 0 | A | G | 61.1% | ‑3.8 / 13.6 | 18 | I83V (ATT→GTT) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base A (2/5); new base G (11/0); total (13/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGAT‑AAAATTTCCATCAACTCTCCTGCGCTGGCAGACCC > NZ_CP009273/2089484‑2089653 | gCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGtt > 1:760814/1‑90 (MQ=255) ttCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTaa > 2:717573/1‑90 (MQ=255) tCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAg < 1:234215/90‑1 (MQ=255) tCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAg < 2:781353/90‑1 (MQ=255) gtgtGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGATg > 2:317872/1‑90 (MQ=255) gtgGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGcc < 2:160441/90‑1 (MQ=255) aaaGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTTAGTCTCTGGGAGATGGCGCGaaaaatt > 1:874344/1‑87 (MQ=255) tGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGAAGATGCCGCGAAAAATCttttc > 2:98894/1‑88 (MQ=255) gggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGATGCCGCGAAAAATTTTTTCt > 1:269338/1‑90 (MQ=255) cgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAAATTTTTCCTTTTGGGCggg > 2:433342/1‑89 (MQ=255) gATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGCTCTGGGAGAAGCCCCGGAAAATTTTTTCTTTGGCGCGGGTAAAAATTTcct > 1:800078/1‑89 (MQ=255) aCATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTTTCTGGAAGAAGCCCCGAAAATTTTTTCCTTTTGCGCGGGA‑AAAAATTCCATCAAct > 2:599795/1‑90 (MQ=255) ttGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCGCGAAAAATTTTTTCTTTTGGGCGGGT‑AAAATTTTCATCAACTCTCCTGCGCt > 1:234117/1‑90 (MQ=255) tgGCGGGGGGGGTTAAGTCTCTGGAAGATAGCGCGAAAATTCTTTCCTTTTGCGCGGGT‑AAAAATTTCATCAACTCTCCCTCGCTGGCAg > 2:525240/1‑90 (MQ=255) gCGGGTGGGGTTAAGTGTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGAT‑AAAAATTCCATCAAATCTCCTTCGCCGGgagaa > 2:585664/1‑89 (MQ=255) gCGGGGGGGGTTAAGTCTCTGGAAGATGCCGCGAAAATTTTTTTCTTTTGGGCGGGT‑AAAATTTTCATCAAATCTCCTGCGCTGGCAGAc > 1:317136/1‑90 (MQ=255) gggTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGAT‑AAAATTTCCATCAACTCTCCTGCGCTGGCAGAccc < 2:31021/90‑1 (MQ=255) gggTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGAT‑AAAATTTCCATCAACTCTCCTGCGCTGGCAGAccc < 1:55376/90‑1 (MQ=255) | GCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGAT‑AAAATTTCCATCAACTCTCCTGCGCTGGCAGACCC > NZ_CP009273/2089484‑2089653 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |