| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,471,278 | 0 | G | C | 55.6% | 6.9 / 10.6 | 9 | C193W (TGC→TGG) | slyD | peptidylprolyl isomerase |
| Reads supporting (aligned to +/- strand): ref base G (4/0); new base C (0/5); total (4/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.94e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATT‑CCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGG‑CAA‑CCGCAA‑CCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCG > NZ_CP009273/3471197‑3471362 | aCGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATT‑CCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGG‑CAA‑CCGCAA‑ccgcc > 2:90709/1‑90 (MQ=255) tGAGGCGTAAAAAAAGCGGGGATT‑CCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGG‑CAA‑CCGCAA‑CCGCCGTTGCCTTTACCGCcaca > 2:804668/1‑90 (MQ=255) gaaaaaaaaaGGGGGGTTT‑CCCCCTTTTTTTTTCCTTTTTTTGGTTTTTGGGG‑CAA‑CCCCAACCCGCCGTTGCCTTTACCGCCAcagcag < 2:333856/87‑1 (MQ=255) gaaaaaaaaaGGGGGGCTC‑CCCCCTTTTTTTTTCCTTTTTTCCGTTATTGGGG‑CCA‑CCCCAACCCGCCGTTGCCTTTACCGCCAcagcag < 2:463653/87‑1 (MQ=255) gTAAAAAAAGCGGGGATT‑CCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGG‑CAA‑CCGCAA‑CCGCCGTTGCCTTTACCGCCACAGCAGcc > 2:185176/1‑90 (MQ=255) gTAAAAAAAGCGGGGATT‑CCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGG‑CAA‑CCGCAA‑CCGCCGTTGCCTTTACCGCCACAGCAGcc > 2:82214/1‑90 (MQ=255) aaaaaaaaGGGGGGTAT‑CCCCCTTTTTTTTTTCCTTTTTTGGTTTTTGGGG‑CAACCCCCAC‑CCGCCGTTGCCTTTACCGCCACAGCAGcc < 2:335712/89‑1 (MQ=255) ggggggATTCCCCCTTTTTTTTTTCTTTTTTTCGTTTTTGGGGC‑CAC‑CCCCAC‑CCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCAc < 2:165744/88‑1 (MQ=255) ctttttttGTC‑TTTTTTCGGTTTTTGGGGCCAC‑CCCCAC‑CCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCg < 2:111018/88‑1 (MQ=255) caa‑ccgcaa‑ccgCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACcg > 1:649753/1‑90 (MQ=255) | ACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATT‑CCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGG‑CAA‑CCGCAA‑CCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCG > NZ_CP009273/3471197‑3471362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |