| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,561,000 | 0 | A | C | 55.0% | 15.3 / 25.9 | 20 | V131G (GTG→GGG) | yjiR | PLP‑dependent aminotransferase family protein |
| Reads supporting (aligned to +/- strand): ref base A (7/2); new base C (0/11); total (7/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTA > NZ_CP009273/4560912‑4561089 | gAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGcaccac > 2:285037/1‑90 (MQ=255) aGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCAccc < 2:35274/85‑1 (MQ=255) aGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCAccc > 1:35274/1‑85 (MQ=255) gCTCCCGCAATCCTCGCTGCCCGGCTAAAAAGTCATACCCGAGAACGGTTTGCAGATTATGCTGCCCCCCCCGGCTTAGCTCCCGCcaca < 1:714887/90‑1 (MQ=255) tctcGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCCCCCGGCTTAGCTCCCGCCACAGc < 2:118109/90‑1 (MQ=255) aaTACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCCCCCGGCTTAGCTCCCGCCACAGCGGTTTc < 1:890088/90‑1 (MQ=255) tACTCGCTTACCGGCTAACAAGTCATAACCTGGAACGGTTTGCAGATTATGCTTCACCACCCGGCTTAGCTCCCGCCACAGGGGTGTCAg > 1:566003/1‑90 (MQ=255) aCCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCCCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGgcgc < 2:79035/90‑1 (MQ=255) cGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCCCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCtt < 2:82635/90‑1 (MQ=255) ggCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTc > 1:399410/1‑90 (MQ=255) gCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCg > 1:755739/1‑90 (MQ=255) aTAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGgtgt < 1:285037/90‑1 (MQ=255) aCCGAGAACGGTTTGCAGTTTATGCTGCCCCCCCCGGTTTAGCCCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCt < 1:296832/90‑1 (MQ=255) agaACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTg > 1:456487/1‑90 (MQ=255) gaACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGc > 2:22877/1‑90 (MQ=255) gTTTGCAGATTATGCTGCACCCCCCGGCTTAGCCCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAAc < 2:621530/90‑1 (MQ=255) tGCAGATTATGCTGCACCCCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGa < 2:46633/90‑1 (MQ=255) tGCAGATTATGCTCCCCCCCCCGGTTTAGCCCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGa < 1:499661/90‑1 (MQ=255) aTTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAAt > 1:139070/1‑90 (MQ=255) ttATGCTGCCCCCCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATg < 1:141301/90‑1 (MQ=255) aTGCTACACCCCCCGGCTTACCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGa < 2:399410/90‑1 (MQ=255) accCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTa > 2:163475/1‑90 (MQ=255) accCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTa > 2:52525/1‑90 (MQ=255) | GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTA > NZ_CP009273/4560912‑4561089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |