| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,903 | T→A | D27E (GAT→GAA) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,903 | 0 | T | A | 97.5% | 133.8 / ‑5.4 | 40 | D27E (GAT→GAA) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (18/21); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCA > NZ_CP009273/49820‑49987 | tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc < 2:252530/90‑1 (MQ=255) cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcca < 1:456711/90‑2 (MQ=255) cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa > 1:218705/1‑90 (MQ=255) cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTaa > 2:795128/1‑90 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc < 2:436021/81‑1 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc > 1:436021/1‑81 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCaa < 1:827998/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCaa < 2:47646/90‑1 (MQ=255) aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAac < 1:238508/90‑1 (MQ=255) aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAac > 1:92375/1‑90 (MQ=255) aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAac < 1:318354/90‑1 (MQ=255) aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAac < 1:667745/90‑1 (MQ=255) aTTGCGGCGTTAGCGGTAGATCGCGTTATCGCCATGGAAAACACCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAac < 1:572397/90‑1 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcca > 1:270691/1‑70 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcca < 2:270691/71‑2 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAACACCtt > 2:704058/1‑90 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAACACCtt > 2:704592/1‑90 (MQ=255) tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAACACCTtaaata < 2:807634/90‑1 (MQ=255) gcgTTATCGGCATGGAAAACGCCCTGCCGGGGAACCTGCCTGCCCAACTCGCCAGGGTTAAAAGCAAAACCTTAAATAAACCCGGGATTa > 2:702727/1‑90 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGGACTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa > 2:872875/1‑90 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCaa < 2:123371/67‑1 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCaa > 1:123371/1‑67 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGGTTAAACGCAACACCTTAAATAAACCCGTGATTa > 1:614736/1‑90 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCGGCCTGCCGAACTCGCCCGGGTTAAACGCAAAACCTTAAATAAACCCCTGATTa > 1:862355/1‑90 (MQ=255) cgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTAt > 1:179450/1‑90 (MQ=255) ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCaa > 2:5491/1‑64 (MQ=255) ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCaa < 1:5491/64‑1 (MQ=255) aTCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc < 2:92375/90‑1 (MQ=255) cATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa < 2:460768/90‑1 (MQ=255) aTGGAAACCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt < 1:702727/90‑1 (MQ=255) aTGGAAACCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt < 1:587290/90‑1 (MQ=255) aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt > 1:479641/1‑90 (MQ=255) tGGAAAACGCCATGCCGTGGACCCTGCCTGCCGAACTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATa < 1:639210/90‑1 (MQ=255) cATGCCGTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatc > 2:553807/1‑90 (MQ=255) gTGGAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg < 1:872875/90‑1 (MQ=255) ggAACCTGCCTGCCGAACTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc < 1:245875/90‑1 (MQ=255) tgcctgccGAACTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc < 2:346723/84‑1 (MQ=255) tgcctgccGAACTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc > 1:346723/1‑84 (MQ=255) gcctgccGAACTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGtt > 1:654516/1‑90 (MQ=255) gccGAACTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCa > 2:183819/1‑90 (MQ=255) | TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCA > NZ_CP009273/49820‑49987 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |