Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,632,461	T→G	L487R (CTT→CGT)	pitA →	inorganic phosphate transporter PitA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,632,461	0	T	G	100.0%	186.7 / NA	48	L487R (CTT→CGT)	pitA	inorganic phosphate transporter PitA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/24);  total (24/24)

GGTAGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTG  >  NZ_CP009273/3632378‑3632546                                                                                    |                                                                                      ggtAGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCcgg                                                                                 <  2:34993/90‑1 (MQ=255) ggtAGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCcgg                                                                                 <  1:210717/90‑1 (MQ=255)     gATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCggg                                                                             <  2:661042/90‑1 (MQ=255)      aTGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                            >  2:286516/1‑90 (MQ=255)       tggtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                            <  1:163608/89‑1 (MQ=255)       tggtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                            >  2:163608/1‑89 (MQ=255)        ggtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTg                                                                          >  1:363511/1‑90 (MQ=255)         gtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGt                                                                         >  1:255475/1‑90 (MQ=255)              ggcTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTgg                                                                    >  2:46260/1‑90 (MQ=255)                cTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGct                                                                  <  2:267760/90‑1 (MQ=255)                cTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGct                                                                  <  1:46210/90‑1 (MQ=255)                cTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGCCGGGCTGTACTGGct                                                                  <  1:435263/90‑1 (MQ=255)                            aaCCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGtt                                                      <  2:508387/90‑1 (MQ=255)                                gTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGtt                                                      <  2:796091/86‑1 (MQ=255)                                gTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGtt                                                      >  1:796091/1‑86 (MQ=255)                                gTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTg                                                  >  2:470988/1‑90 (MQ=255)                                  gACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCt                                                                           <  2:596143/63‑1 (MQ=255)                                  gACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACGGCGTTCCGGCGGGCt                                                                           >  1:596143/1‑63 (MQ=255)                                         aTTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTAc                                         <  1:471891/90‑1 (MQ=255)                                         aTTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTAc                                         <  1:208012/90‑1 (MQ=255)                                          ttCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACg                                        <  2:614656/90‑1 (MQ=255)                                             tGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                            >  2:839704/1‑51 (MQ=255)                                             tGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                            <  1:839704/51‑1 (MQ=255)                                              gATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCAcc                                    >  1:180973/1‑90 (MQ=255)                                                 ggCCTGGGTGTTTACCGTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCaaa                                 >  1:675407/1‑90 (MQ=255)                                                 ggCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                            >  2:51394/1‑47 (MQ=255)                                                 ggCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                            <  1:51394/47‑1 (MQ=255)                                                 ggCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCaaa                                 >  2:654751/1‑90 (MQ=255)                                                 ggCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCaaa                                 >  1:309330/1‑90 (MQ=255)                                                 ggCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCaaa                                 >  1:490634/1‑90 (MQ=255)                                                       ggTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGc                           <  1:277506/90‑1 (MQ=255)                                                       ggTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGc                           <  1:470988/90‑1 (MQ=255)                                                          gtTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCggg                        <  2:196904/90‑1 (MQ=255)                                                            ttACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                            >  2:314648/1‑36 (MQ=38)                                                            ttACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                            <  1:314648/36‑1 (MQ=38)                                                            ttACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTc                      >  2:846930/1‑90 (MQ=255)                                                            ttACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTc                      >  2:273797/1‑90 (MQ=255)                                                                  ttCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGtt                                                      <  2:570550/52‑1 (MQ=255)                                                                  ttCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGtt                                                      >  1:570550/1‑52 (MQ=255)                                                                  ttCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTgg                >  2:378741/1‑90 (MQ=255)                                                                  ttCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTgg                >  1:803479/1‑90 (MQ=255)                                                                  ttCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTgg                >  2:846329/1‑90 (MQ=255)                                                                    ccGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCggg                        <  2:173671/80‑1 (MQ=255)                                                                    ccGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCggg                        >  1:173671/1‑80 (MQ=255)                                                                      ggCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCg            >  2:448878/1‑90 (MQ=255)                                                                      ggCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCg            <  1:364882/90‑1 (MQ=255)                                                                            ggTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAg      <  1:335023/90‑1 (MQ=255)                                                                                cTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATtg  <  1:268366/90‑1 (MQ=255)                                                                                    |                                                                                      GGTAGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTG  >  NZ_CP009273/3632378‑3632546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑