| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 510,067 | 0 | C | T | 52.9% | 8.1 / 21.8 | 17 | K83K (AAG→AAA) | ybbJ | NfeD family protein |
| Reads supporting (aligned to +/- strand): ref base C (8/0); new base T (0/9); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCT > NZ_CP009273/509982‑510148 | cgcgCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCtttt > 2:604217/1‑90 (MQ=255) gaTTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGAc > 2:656700/1‑90 (MQ=255) gaTTCCAGCAAAAACCGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGTTTTTGTTCGCGCCCCCGCCGCGAc < 2:98308/90‑1 (MQ=255) ttCCAGCAAAAACCGTCGGCCAATCAGCTGCTCCCCGCGCTGGTTTAAATGACTATCGCTGTGTTTTTGTTCGCGCCCCCGCCGCGACaa < 2:186631/90‑1 (MQ=255) cacaacaAACCGCCGCCCAACCACCTGCTCCCCGCGCTGTTTAAAATGACTATCGCTGTGTTTTTGTCCGCCCCCCCGCCGCGACAACCa < 2:56296/86‑1 (MQ=255) cAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCa > 1:56296/1‑90 (MQ=255) cAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCa > 2:691824/1‑90 (MQ=255) cAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCa > 1:667114/1‑90 (MQ=255) cAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCa > 2:17290/1‑90 (MQ=255) cAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCa > 2:367503/1‑90 (MQ=255) acaAACCGCCGGCAAACCAGCTGCTGCCCGCGCTGTTTAAAATGACTATCGCTGTGTTTTTGTTCGCGCCCCCGCCGCGACAACCATTTc < 2:216686/90‑1 (MQ=255) gcccgcCCGCGTTGTTTAAAATGCCTCTCGTTGTTTTTTTGTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGagc < 1:583513/86‑1 (MQ=255) ccGCGCTGTTTAAAATGCTTTTCGTTTTTTTTTTTTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTc < 1:226109/90‑1 (MQ=255) ttaaaaTGCCTCTCGCTGTGTTTTTGCCCGCCCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCa < 1:656828/87‑1 (MQ=255) ttaaaaTGACTACCGCTGTGTTTTTGTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCa < 2:667114/87‑1 (MQ=255) aTCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTAcccc > 2:285123/1‑90 (MQ=255) cccgtgtgTTTTGGTCCCCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCt < 1:248747/86‑1 (MQ=255) | CGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCT > NZ_CP009273/509982‑510148 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |