| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 510,080 | 0 | A | C | 55.6% | 13.2 / 21.3 | 18 | V79G (GTG→GGG) | ybbJ | NfeD family protein |
| Reads supporting (aligned to +/- strand): ref base A (7/1); new base C (0/10); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.11e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGC > NZ_CP009273/510002‑510166 | gaTTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGAc > 2:656700/1‑90 (MQ=255)gaTTCCAGCAAAAACCGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGTTTTTGTTCGCGCCCCCGCCGCGAc < 2:98308/90‑1 (MQ=255) ttCCAGCAAAAACCGTCGGCCAATCAGCTGCTCCCCGCGCTGGTTTAAATGACTATCGCTGTGTTTTTGTTCGCGCCCCCGCCGCGACaa < 2:186631/90‑1 (MQ=255) cacaacaAACCGCCGCCCAACCACCTGCTCCCCGCGCTGTTTAAAATGACTATCGCTGTGTTTTTGTCCGCCCCCCCGCCGCGACAACCa < 2:56296/86‑1 (MQ=255) cAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCa > 1:56296/1‑90 (MQ=255) cAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCa > 1:667114/1‑90 (MQ=255) cAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCa > 2:17290/1‑90 (MQ=255) cAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCa > 2:367503/1‑90 (MQ=255) cAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCa > 2:691824/1‑90 (MQ=255) acaAACCGCCGGCAAACCAGCTGCTGCCCGCGCTGTTTAAAATGACTATCGCTGTGTTTTTGTTCGCGCCCCCGCCGCGACAACCATTTc < 2:216686/90‑1 (MQ=255) gcccgcCCGCGTTGTTTAAAATGCCTCTCGTTGTTTTTTTGTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGagc < 1:583513/86‑1 (MQ=255) ccGCGCTGTTTAAAATGCTTTTCGTTTTTTTTTTTTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTc < 1:226109/90‑1 (MQ=255) ttaaaaTGCCTCTCGCTGTGTTTTTGCCCGCCCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCa < 1:656828/87‑1 (MQ=255) ttaaaaTGACTACCGCTGTGTTTTTGTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCa < 2:667114/87‑1 (MQ=255) aTCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTAcccc > 2:285123/1‑90 (MQ=255) cccgtgtgTTTTGGTCCCCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCt < 1:248747/86‑1 (MQ=255) tttttttCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTc < 1:17290/89‑1 (MQ=255) cgcCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGc < 1:285123/90‑1 (MQ=255) | GATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGC > NZ_CP009273/510002‑510166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |