| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 832,042 | 0 | C | G | 52.6% | 11.3 / 29.0 | 19 | A79G (GCG→GGG) | hcxB | hydroxycarboxylate dehydrogenase HcXB |
| Reads supporting (aligned to +/- strand): ref base C (1/8); new base G (10/0); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATC > NZ_CP009273/831954‑832125 | aTTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGggcg > 2:173605/1‑90 (MQ=255) gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGcgggggcggggggccc > 2:403040/1‑86 (MQ=255) gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCAc > 2:698360/1‑90 (MQ=255) gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCAc > 2:564377/1‑90 (MQ=255) gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCAc > 2:307730/1‑90 (MQ=255) gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCAc > 2:443720/1‑90 (MQ=255) cAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGTCACGcccc > 2:6942/1‑87 (MQ=255) cGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATcgc < 1:6942/90‑1 (MQ=255) cGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATcgc < 2:204697/90‑1 (MQ=255) cTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACGCTCCATGGGGATCgcgc > 2:443121/1‑90 (MQ=255) cTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACCCTCCGTGGCGGTCgcgc > 2:532543/1‑90 (MQ=255) cTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCTCGATGGGGGTCgcgc > 2:16904/1‑90 (MQ=255) ccTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCAt < 1:16904/90‑1 (MQ=255) cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCCCCCCCGAAGGCGCTCGCACCTTTTGTccg > 2:493140/1‑88 (MQ=255) cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCCCCCTGGGGGACGCGCATTTTGTTAg > 1:693007/1‑90 (MQ=255) cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACCCCCCCTGGCGATCGCGCCTTTTGTccg > 1:487376/1‑88 (MQ=255) aCCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCgg < 2:631911/90‑1 (MQ=255) agagGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAgc < 1:443720/90‑1 (MQ=255) agagGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAgc < 2:579367/90‑1 (MQ=255) agagGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAgc < 2:693007/90‑1 (MQ=255) cGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATc < 2:286156/90‑1 (MQ=255) | ATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATC > NZ_CP009273/831954‑832125 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |