Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,725,0550TG54.5% 8.2 / 13.0 11V571G (GTG→GGG) lhrATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base G (6/0);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGACATTATCACTCTCGGCGCTACCTCATGGC  >  NZ_CP009273/1724970‑1725129
                                                                                     |                                                                          
gTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCgggggggg                                                                        >  2:264835/1‑89 (MQ=255)
   aCCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGaa                                                                     <  1:264835/90‑1 (MQ=255)
   aCCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGaa                                                                     <  2:19219/90‑1 (MQ=255)
                  aTTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAAATGGGTGAAgagagg                                                      >  2:46945/1‑88 (MQ=255)
                            gTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGTGAACTGGATGAGGAGATGGGATATGAGt                                            >  2:77524/1‑90 (MQ=255)
                                        gCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGGACTGGATGAGGGGGAGGGATATGAGTCGCGGGGGGACg                                >  1:538419/1‑90 (MQ=255)
                                            gtTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGGACTGGGTGAGGGGGTGGGATATGAGGCGCGGGGGGAAGACAt                            >  1:179328/1‑90 (MQ=255)
                                                  aCCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAACTGGATGAGGAGATGGGATATGAGGCGCGGGGGGACGACATTTTCAc                      >  1:148694/1‑90 (MQ=255)
                                                      gAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATTTGAGTCGCGGGTGAACGACATTATCACtctc                  <  2:538419/90‑1 (MQ=255)
                                                       aaGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAACTGGATGAGGAGATGGGATATGAGGCGCGGGGGAAAGACATTATCACTCTCg                 >  2:367051/1‑90 (MQ=255)
                                                             aagaaAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGACATTATCACTCTCGGCGCTa           <  1:248211/90‑1 (MQ=255)
                                                                      ccGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGACATTATCACTCTCGGCGCTACCTCATGGc  <  1:367051/90‑1 (MQ=255)
                                                                                     |                                                                          
GTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGACATTATCACTCTCGGCGCTACCTCATGGC  >  NZ_CP009273/1724970‑1725129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑