| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,837,171 | 0 | A | G | 57.1% | 1.5 / 12.3 | 14 | M182V (ATG→GTG) | gdhA | NADP‑specific glutamate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (0/6); new base G (8/0); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTC‑A‑CC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTAT > NZ_CP009273/1837091‑1837244 | aTCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGATGaaaa > 2:662717/1‑90 (MQ=255) gCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGATCaaaaaac > 2:694530/1‑88 (MQ=255) cccTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGctct < 2:671342/89‑1 (MQ=255) aCCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGgatg < 2:603214/77‑1 (MQ=255) aCCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGctct < 1:694530/90‑1 (MQ=255) gATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGGTGAAAAAAATCTCccacaaaaac > 2:499113/1‑86 (MQ=255) ggCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTC‑A‑c < 1:570243/90‑1 (MQ=255) gTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTC‑A‑CC‑GGTa < 2:590728/90‑1 (MQ=255) ggggTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTC‑A‑CC‑GGTAAGGGCCtt < 1:662717/90‑1 (MQ=255) ggTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGATGGAAAAAATCTCCCACAAAAACCCCCGCGTCTTT‑C‑CC‑CGGAAGGGGCtttt > 2:508064/1‑89 (MQ=255) tggtggTCGTGAAGTCGGCTTTATGGCGGGGGTGGGGAAAAAAATCCCCAACAAAACCCCCCTCGTCTTC‑ACCC‑GGAAGGGGCCtttttt > 2:585826/1‑87 (MQ=255) ggtggtCGTGAAGTCGGCTTTATGGCGGGGGTGGTGGAAAAAATCTCCCACAAAAACCCCCGCGTCTTT‑T‑CCGGGGAAGGGCtttttttt > 2:87977/1‑86 (MQ=255) ggtggtCGTGAAGTCGGCTTTATGGCGGGGGGGAGGAAAAAACTCTCCCACAAAAACCCCCCCGTCTTCTC‑CC‑GGAAAGGGCGttttttt > 2:421345/1‑86 (MQ=255) cGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTC‑A‑CC‑GGTAAGGGCCTTTCATTTggcggc < 1:499113/90‑1 (MQ=255) cGGCTTTATGGCGGGGGTGGTGGAAAAACTCTCCCACAAAAACGCCCGCGTCCTC‑C‑CC‑CGGAAGGGGCCTTTTTTTTGCGGGAGGCtttt > 2:475060/1‑88 (MQ=255) | ATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTC‑A‑CC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTAT > NZ_CP009273/1837091‑1837244 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |