| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,571 | 0 | A | G | 53.8% | 4.4 / 13.2 | 13 | I83V (ATT→GTT) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base A (0/6); new base G (7/0); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGAC > NZ_CP009273/2089489‑2089651 | cAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGtc < 2:502738/90‑1 (MQ=255) tAGATAAAAGCTGGGTATCTCGCGTGGTGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAATTTCTGGGAGATGCCGC‑Ga > 2:556604/1‑90 (MQ=255) gCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGGCCC‑GAAAAATtttt > 2:611194/1‑90 (MQ=255) gggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTa < 1:49556/57‑1 (MQ=255) gggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTa > 2:49556/1‑57 (MQ=255) cgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCGTTGGGAAATGCCGC‑GAAAAATCTTTTCTTTTGGGCggg > 1:356904/1‑89 (MQ=255) aaGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGCTCTAGAAGATGCCCC‑GAAAATTTTTTTCTTTTGCGCGGGTAAAAatt > 2:515906/1‑90 (MQ=255) tGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCa < 1:108709/90‑1 (MQ=255) tGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCa < 1:514697/90‑1 (MQ=255) aTCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATc < 2:543074/90‑1 (MQ=255) ttCCGTTTTGTGTGGCGGGGGGGGGTAAATCTCTGGGAGATGCCGC‑GAAAAATTTTTTCTTTGGGGCGGGGAAAATTTCCATCAACtctc > 1:430846/1‑90 (MQ=255) ttttGTGTGGCGGGGGGGGGTTAGTCTCTGGGAGAAGCCGCGGAAAATTTTTTCTTTTGG‑GCGGGTAAAAATTTCATCCACTCTCCTgcg > 1:323171/1‑90 (MQ=255) gCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGAc < 2:323171/90‑1 (MQ=255) | CAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGAC > NZ_CP009273/2089489‑2089651 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |