| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,254,480 | 0 | A | C | 52.9% | 15.8 / 24.1 | 17 | V137G (GTG→GGG) | fruA | PTS fructose transporter subunit IIBC |
| Reads supporting (aligned to +/- strand): ref base A (8/0); new base C (0/9); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTT‑CA‑CCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGTCGGGCAAGCAG > NZ_CP009273/2254420‑2254561 | gcGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTT‑CA‑CCCACCAGCCACGTTTTTTCGCTTCGGtt > 2:25030/1‑90 (MQ=255) gTGATTGCATTCCCCGCGCCAACAGAACCACGGGTTTCAACTTT‑CC‑CCCCCCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgcc < 2:216069/90‑1 (MQ=255) gTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTT‑CC‑CCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgcc < 2:87938/90‑1 (MQ=255) gTGATTCCTTTCCCCGCCCCAACAGACCCGGGGTTTTCAATTTC‑CC‑CCCCCCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgcc < 1:319588/90‑1 (MQ=255) gATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTT‑CA‑CCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgccgc > 2:372066/1‑90 (MQ=255) gATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTT‑CA‑CCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgccgc > 2:620715/1‑90 (MQ=255) aCCCGCGCCAACAGAACCACGGGTTTCAACTTT‑ca‑cccacc > 1:654395/1‑41 (MQ=255) aCCCGCGCCAACAGAACCACGGGTTTCAACTTT‑ca‑cccacc < 2:654395/41‑1 (MQ=255) ccgcCAACAG‑ACCCCGGGTTTTCACTTTCCC‑CCCCCCAGCCCCTTTTTTTCGCTTCGGTTTCAAGGGCTTCAGCCGCCATAAAGgtgtgt < 1:563422/89‑1 (MQ=255) cgcCAACAGAACCACGGGTTTCAACTTT‑CA‑CCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGtgtgtg > 1:551427/1‑90 (MQ=255) cgcCAACAGAACCACGGGTTTCAACTTT‑CA‑CCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGtgtgtg > 2:412369/1‑90 (MQ=255) gcCAACAGAACCACGGGTTTCAACTTT‑CC‑CCCCCCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGc < 2:160537/90‑1 (MQ=255) cAGACCCCCGGTTTTCAACTTC‑CC‑CCCCCCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGc < 2:693744/90‑1 (MQ=255) aCCACGGGTTTCAACTTT‑CA‑CCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGt > 1:569806/1‑90 (MQ=255) aaCTTT‑CCCCCCCCCAGCCTCTTTTTTTCGTTTGGGTTTAAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGTCGGGCAAGCAg < 1:630881/90‑1 (MQ=255) tt‑CC‑CCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgccgc < 1:698657/50‑1 (MQ=255) tt‑CC‑CCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGTCGGGCAAGCAg < 2:500606/85‑1 (MQ=255) tt‑CA‑CCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgccgc > 2:698657/1‑50 (MQ=255) tt‑CA‑CCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGTCGGGCAAGCAg > 1:500606/1‑85 (MQ=255) | GCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTT‑CA‑CCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGTCGGGCAAGCAG > NZ_CP009273/2254420‑2254561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |