Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,738,4480AC54.5% 11.6 / 24.8 23V84G (GTG→GGG) trmDtRNA (guanosine(37)‑N1)‑methyltransferase TrmD
Reads supporting (aligned to +/- strand):  ref base A (7/3);  new base C (0/12);  total (7/16)
Fisher's exact test for biased strand distribution p-value = 7.04e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-04
Rejected as consensus: Frequency below/above cutoff threshold.

CACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCC  >  NZ_CP009273/2738366‑2738533
                                                                                  |                                                                                     
cacCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTgc                                                                                <  2:155492/90‑1 (MQ=255)
       aTCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCa                                                                         >  2:272000/1‑90 (MQ=255)
         cAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCAcc                                                                       <  2:457683/90‑1 (MQ=255)
            tttttGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACccgc                                                                    >  2:482725/1‑90 (MQ=255)
               ttGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCcgccgc                                                                 >  1:408300/1‑90 (MQ=255)
                      gTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCtttt                                                          <  2:381588/90‑1 (MQ=255)
                             gTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCAt                                                   >  1:125838/1‑90 (MQ=255)
                                            tttGACCAAGCTTGCGTCCTGGTGGGGAAAGATAAATCCCCTTTGCCCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                    <  1:441893/89‑1 (MQ=255)
                                            cTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                    <  2:378305/90‑1 (MQ=255)
                                            cTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                    <  2:48054/90‑1 (MQ=255)
                                             ttGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCaa                                   <  2:218917/90‑1 (MQ=255)
                                             ttGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCaa                                   <  1:272000/90‑1 (MQ=255)
                                               gATCAAGCTTGCGTCCTTGTGGTGACAGATAAACCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAgg                                 <  1:416184/90‑1 (MQ=255)
                                                 tCAAGCTTGCGTCCCTGTGGTGACAGAAAAACCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGt                               <  1:482725/90‑1 (MQ=255)
                                                      cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  1:100574/90‑1 (MQ=255)
                                                       ttGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAcc                         <  2:646945/90‑1 (MQ=255)
                                                        tGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACca                        <  2:283716/90‑1 (MQ=255)
                                                              ccTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTa                  >  1:269429/1‑90 (MQ=255)
                                                                      gaaaGATAAACCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATcccc          <  2:477881/87‑1 (MQ=255)
                                                                      gACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATcccc          >  1:541679/1‑90 (MQ=255)
                                                                      gACAAAAAACTCTCCTTTGCTCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATcccc          <  2:533537/90‑1 (MQ=255)
                                                                           aTAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTcc     >  1:667899/1‑90 (MQ=255)
                                                                              aaTCCCCTTTGCGCTTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgcc  <  2:408300/90‑1 (MQ=255)
                                                                                  |                                                                                     
CACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCC  >  NZ_CP009273/2738366‑2738533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: